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Journal Articles Cold Spring Harbor protocols Year : 2013

Combining calcium imaging with other optical techniques.

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Abstract

Ca(2+) imaging is a commonly used approach for measuring Ca(2+) signals at high spatial resolution. The method is often combined with electrode recordings to correlate electrical and chemical signals or to investigate Ca(2+) signals following an electrical stimulation. To obtain information on electrical activity at the same spatial resolution, Ca(2+) imaging must be combined with membrane potential imaging. Similarly, stimulation of subcellular compartments requires photostimulation. Thus, combining Ca(2+) imaging with an additional optical technique facilitates the study of a number of physiological questions. The aim of this article is to introduce some basic principles regarding the combination of Ca(2+) imaging with other optical techniques. We discuss the design of the optics, the design of experimental protocols, the optical characteristics of Ca(2+) indicators used in combination with an optical probe, and the affinity of the Ca(2+) indicator in relation to the type of measurement. This information will enable the reader to devise an optimal strategy for combined optical experiments.
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Dates and versions

inserm-00951425 , version 1 (24-02-2014)

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Marco Canepari, Dejan Zecevic, Kaspar E. Vogt, David Ogden, Michel de Waard. Combining calcium imaging with other optical techniques.: Ca 2+ Imaging and Other Optical Techniques. Cold Spring Harbor protocols, 2013, 2013 (12), pp.1125-31. ⟨10.1101/pdb.top066167⟩. ⟨inserm-00951425⟩
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