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A small RNA controls a protein regulator involved in antibiotic resistance in Staphylococcus aureus.

Abstract : The emergence of Staphylococcus aureus strains that are resistant to glycopeptides has led to alarming scenarios where serious staphylococcal infections cannot be treated. The bacterium expresses many small regulatory RNAs (sRNAs) that have unknown biological functions for the most part. Here we show that an S. aureus sRNA, SprX (alias RsaOR), shapes bacterial resistance to glycopeptides, the invaluable treatments for Methicillin-resistant staphylococcal infections. Modifying SprX expression levels influences Vancomycin and Teicoplanin glycopeptide resistance. Comparative proteomic studies have identified that SprX specifically downregulates stage V sporulation protein G, SpoVG. SpoVG is produced from the yabJ-spoVG operon and contributes to S. aureus glycopeptide resistance. SprX negatively regulates SpoVG expression by direct antisense pairings at the internal translation initiation signals of the second operon gene, without modifying bicistronic mRNA expression levels or affecting YabJ translation. The SprX and yabJ-spoVG mRNA domains involved in the interaction have been identified, highlighting the importance of a CU-rich loop of SprX in the control of SpoVG expression. We have shown that SpoVG might not be the unique SprX target involved in the glycopeptide resistance and demonstrated that the regulation of glycopeptide sensitivity involves the CU-rich domain of SprX. Here we report the case of a sRNA influencing antibiotic resistance of a major human pathogen.
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Submitted on : Monday, February 24, 2014 - 10:24:15 AM
Last modification on : Wednesday, March 27, 2019 - 3:14:52 PM
Long-term archiving on: : Saturday, May 24, 2014 - 10:55:16 AM

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Alex Eyraud, Pierre Tattevin, Svetlana Chabelskaya, Brice Felden. A small RNA controls a protein regulator involved in antibiotic resistance in Staphylococcus aureus.. Nucleic Acids Research, Oxford University Press, 2014, 42 (8), pp.4892-905. ⟨10.1093/nar/gku149⟩. ⟨inserm-00951043⟩

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