In vivo germination of Bacillus anthracis spores during murine cutaneous infection. - Archive ouverte HAL Access content directly
Journal Articles Journal of Infectious Diseases Year : 2013

In vivo germination of Bacillus anthracis spores during murine cutaneous infection.

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Abstract

BACKGROUND: Germination is a key step for successful Bacillus anthracis colonization and systemic dissemination. Few data are available on spore germination in vivo, and the necessity of spore and host cell interactions to initiate germination is unclear. METHODS: To investigate the early interactions between B. anthracis spores and cutaneous tissue, spores were inoculated in an intraperitoneal cell-free device in guinea pigs or into the pinna of mice. Germination and bacterial growth were analyzed through colony-forming unit enumeration and electron microscopy. RESULTS: In the guinea pig model, germination occurred in vivo in the absence of cell contact. Similarly, in the mouse ear, germination started within 15 minutes after inoculation, and germinating spores were found in the absence of surrounding cells. Germination was not observed in macrophage-rich draining lymph nodes, liver, and spleen. Edema and lethal toxin production were not required for germination, as a toxin-deficient strain was as effective as a Sterne-like strain. B. anthracis growth was locally controlled for 6 hours. CONCLUSIONS: Spore germination involving no cell interactions can occur in vivo, suggesting that diffusible germinants or other signals appear sufficient. Different host tissues display drastic differences in germination-triggering capacity. Initial control of bacterial growth suggests a therapeutic means to exploit host innate defenses to hinder B. anthracis colonization.

Dates and versions

inserm-00926617 , version 1 (09-01-2014)

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Jean-Philippe Corre, Alejandro Piris-Gimenez, Marie Moya-Nilges, Gregory Jouvion, Agnes Fouet, et al.. In vivo germination of Bacillus anthracis spores during murine cutaneous infection.. Journal of Infectious Diseases, 2013, 207 (3), pp.450-7. ⟨10.1093/infdis/jis686⟩. ⟨inserm-00926617⟩
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