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IL-22 is produced by γC-independent CD25+ CCR6+ innate murine spleen cells upon inflammatory stimuli and contributes to LPS-induced lethality.

Abstract : IL-22 is a Th17 cytokine that plays a key role in immune responses against extracellular bacteria. In mucosal lymphoid tissues, IL-22 production is mainly due to an IL-23-responsive NK-like cell subset that shares some markers with lymphoid tissue inducer (LTi) cells. Here, we identified a new spleen cell population responsible for IL-22 production upon either in vitro stimulation by anti-CD3 antibodies or in vivo stimulation by lipopolysaccharide (LPS) via IL-2- and an IL-23-dependent mechanisms, respectively. These cells represent 1% of spleen cells from recombination activating gene (Rag2)-deficient mice, and correspond to a discrete innate lymphoid cell population expressing CD25, CCR6 and IL-7R. This population comprises 60-70% CD4(+) cells, which produce IL-22, and are still present in common γ chain-deficient mice; the CD4(-) subset coexpresses IL-22 and IL-17, and is common γ chain-dependent. The importance of IL-22 production for the LPS-triggered response is highlighted by the fact that IL-22-deficient mice are more resistant to LPS-induced mortality.
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https://www.hal.inserm.fr/inserm-00926611
Contributor : Jean-Claude Sirard <>
Submitted on : Thursday, January 9, 2014 - 9:41:19 PM
Last modification on : Thursday, April 9, 2020 - 1:47:45 AM

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Laure Dumoutier, Magali de Heusch, Ciriana Orabona, Naoko Satoh-Takayama, Gerard Eberl, et al.. IL-22 is produced by γC-independent CD25+ CCR6+ innate murine spleen cells upon inflammatory stimuli and contributes to LPS-induced lethality.. European Journal of Immunology, Wiley-VCH Verlag, 2011, 41 (4), pp.1075-85. ⟨10.1002/eji.201040878⟩. ⟨inserm-00926611⟩

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