Simultaneous determination of five synthetic pyrethroid metabolites in urine by liquid chromatography-tandem mass spectrometry: application to 39 persons without known exposure to pyrethroids.

R. Le Grand 1 S. Dulaurent 1 J. M. Gaulier 1 Franck Saint-Marcoux 1, 2 Christian Moesch 1 G. Lachâtre 1
1 Service de Pharmacologie, toxicologie et pharmacovigilance [CHU Limoges]
2 PIST - Pharmacologie des Immunosuppresseurs et de la Transplantation
Abstract : A sensitive and reliable method was developed and validated for the determination of five synthetic pyrethroid metabolites namely cis-Cl(2)CA, trans-Cl(2)CA, Br(2)CA, 3-PBA and 4-FPBA in human urine by liquid chromatography-tandem mass spectrometry. (2)D(6)-labelled trans-Cl(2)CA and (13)C(6)-labelled 3-PBA were used as internal standards. This method was based on a liquid-liquid extraction procedure in acidic conditions using hexane solvent with a basic purification, a chromatographic separation using a specific C18 column and mass spectrometric detection in the negative polarity. Suitable limits of detection (0.015μg/L for the five compounds) and quantification (from 0.020 to 0.030μg/L) were obtained for rendering the method usable for the biomonitoring of pyrethroids in the general population. The efficiency of the method was tested in 39 urine samples from French people without any known exposure to pyrethroids. At least three of the five metabolites were detected in each sample. The results of this study were compared to those obtained in previous ones and discussed.
Type de document :
Article dans une revue
Toxicology Letters, Elsevier, 2012, 210 (2), pp.248-53. 〈10.1016/j.toxlet.2011.08.016〉
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http://www.hal.inserm.fr/inserm-00926148
Contributeur : Pierre Marquet <>
Soumis le : jeudi 9 janvier 2014 - 10:40:08
Dernière modification le : vendredi 16 février 2018 - 15:25:00

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UNILIM | UMR-S850 | GEIST | IPPRITT

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R. Le Grand, S. Dulaurent, J. M. Gaulier, Franck Saint-Marcoux, Christian Moesch, et al.. Simultaneous determination of five synthetic pyrethroid metabolites in urine by liquid chromatography-tandem mass spectrometry: application to 39 persons without known exposure to pyrethroids.. Toxicology Letters, Elsevier, 2012, 210 (2), pp.248-53. 〈10.1016/j.toxlet.2011.08.016〉. 〈inserm-00926148〉

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