Is biofilm formation related to the hypermutator phenotype in clinical Enterobacteriaceae isolates?

Bela Kovacs 1 Sandrine Le Gall-David 2, 3 Pascal Vincent 2, 3 Hervé Le Bars 2, 3 Sylvie Buffet-Bataillon 2, 3 Martine Bonnaure-Mallet 2, 3 Anne Jolivet-Gougeon 2, 3, *
* Auteur correspondant
1 Department of Urology
2 Microbiologie : Risques Infectieux
3 UEB - Université européenne de Bretagne
Abstract : : In bacteria, complex adaptive processes are involved during transition from the planktonic to the biofilm mode of growth, and mutator strains are more prone to producing biofilms. Enterobacteriaceae species were isolated from urinary tract infections (UTIs; 222 strains) and from bloodstream infections (BSIs; 213 strains). Relationship between the hypermutable phenotype and biofilm forming capacity was investigated in these clinical strains. Mutation frequencies were estimated by monitoring the capacity of each strain to generate mutations that conferred rifampicin resistance on supplemented medium. Initiation of biofilm formation was assayed by determining the ability of the cells to adhere to a 96-well polystyrene microtitre plate. UTI Enterobacteriaceae strains showed significantly higher biofilm-forming capacity: 63.1% (54.0% for E. coli strains) vs. 42.3% for BSI strains (47.7% for E. coli). Strains isolated from UTIs did not present higher mutation frequencies than those from BSIs: contrary to what has been widely described for P. aeruginosa strains, isolated from pulmonary samples in patients suffering from cystic fibrosis, no relationship was found between the hypermutator phenotype in Enterobacteriaceae and the ability to initiate a biofilm.
Keywords : urinary tract infection bloodstream infection hypermutation mutation frequencies
Type de document :
Article dans une revue
FEMS Microbiology Letters, Wiley-Blackwell, 2013, 347 (2), pp.116-122. 〈10.1111/1574-6968.12229〉
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Contributeur : Céline Allaire <>
Soumis le : mardi 7 janvier 2014 - 15:35:25
Dernière modification le : mercredi 16 mai 2018 - 11:23:28

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BIOSIT | NUMECAN | NUMECAN-CIMIAD | UR1-UFR-SVE | UNIV-RENNES

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Bela Kovacs, Sandrine Le Gall-David, Pascal Vincent, Hervé Le Bars, Sylvie Buffet-Bataillon, et al.. Is biofilm formation related to the hypermutator phenotype in clinical Enterobacteriaceae isolates?. FEMS Microbiology Letters, Wiley-Blackwell, 2013, 347 (2), pp.116-122. 〈10.1111/1574-6968.12229〉. 〈inserm-00925055〉

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