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Journal articles
Small molecule activators of the Nrf2-HO-1 antioxidant axis modulate heme metabolism and inflammation in BV2 microglia cells.
Abstract : The nuclear factor erythroid derived 2-related factor 2 (Nrf2) and the antioxidant protein heme oxygenase-1 (HO-1) are crucial components of the cellular stress response. These two systems work together to combat oxidative stress and inflammation and are attractive drug targets for counteracting different pathologies, including neuroinflammation. We aimed to identify the most effective Nrf2/HO-1 activators that modulate the inflammatory response in microglia cells. In the present study, we searched the literature and selected 56 compounds reported to activate Nrf2 or HO-1 and analyzed them for HO-1 induction at 6 and 24h and cytotoxicity in BV2 microglial cells in vitro. Approximately 20 compounds up-regulated HO-1 at the concentrations tested (5-20 μM) with carnosol, supercurcumin, cobalt protoporphyrin-IX and dimethyl fumarate exhibiting the best induction/low cytotoxicity profile. Up-regulation of HO-1 by some compounds resulted in increased cellular bilirubin levels but did not augment the expression of proteins involved in heme synthesis (ALAS 1) or biliverdin reductase. Bilirubin production by HO-1 inducers correlated with their potency in inhibiting nitrite production after challenge with interferon-γ (INF-γ) or lipopolysaccharide (LPS). The compounds down-regulated the inflammatory response (TNF-α, PGE2 and nitrite) more strongly in cells challenged with INF-γ than LPS, and silencing HO-1 or Nrf2 with shRNA differentially affected the levels of inflammatory markers. These findings indicate that some small activators of Nrf2/HO-1 are effective modulators of microglia inflammation and highlight the chemical scaffolds that can serve for the synthesis of potent new derivatives to counteract neuroinflammation and neurodegeneration.
Cited literature [44 references]
https://www.hal.inserm.fr/inserm-00903755
Contributor : Georges Guellaen Connect in order to contact the contributor
Submitted on : Wednesday, November 13, 2013 - 8:10:05 AM
Last modification on : Tuesday, October 19, 2021 - 4:09:20 PM
Long-term archiving on: : Friday, February 14, 2014 - 3:25:23 PM
Contributor : Georges Guellaen Connect in order to contact the contributor
Submitted on : Wednesday, November 13, 2013 - 8:10:05 AM
Last modification on : Tuesday, October 19, 2021 - 4:09:20 PM
Long-term archiving on: : Friday, February 14, 2014 - 3:25:23 PM
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YPHRS-D-13-00198_accepted.pdf
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