A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains. - Archive ouverte HAL Access content directly
Journal Articles Genome Biology Year : 2013

A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains.

(1) , (1) , (2) , (3) , (3) , (1) , (4) , (2) , (4) , (5) , (6) , (6, 7) , (2) , (3) , (2) , (8) , (9) , (10) , (6, 7) , (4) , (11) , (3) , (4) , (2) , (1) , (4) , (3) , (1) , (1) , (1) , (4) , (4) , (2) , (1) , (1) , (2) , (10) , (1) , (3) , (3) , (1) , (6) , (2) , (1) , (12) , (3) , (1) , (13) , (2) , (3) , (2) , (1) , (4) , (2) , (1) , (10) , (2) , (1) , (3, 14, 15, 16) , (5) , (4, 17) , (2) , (2) , (1) , (1) , (4) , (1)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
Tania Sorg
  • Function : Author
  • PersonId : 947137
Kim Wong
  • Function : Author
  • PersonId : 947138
Elodie Bedu
  • Function : Author
  • PersonId : 947139
Elizabeth Cartwright
  • Function : Author
  • PersonId : 947140
Romain Dacquin
  • Function : Author
  • PersonId : 947141
Jochen Graw
  • Function : Author
  • PersonId : 947143
Neil Ingham
  • Function : Author
  • PersonId : 947144
Ian Jackson
  • Function : Author
  • PersonId : 947145
Andreas Lengeling
  • Function : Author
  • PersonId : 947146
Silvia Mandillo
  • Function : Author
  • PersonId : 947147
Hamid Meziane
  • Function : Author
  • PersonId : 947148
Frédéric Preitner
  • Function : Author
  • PersonId : 947149
Oliver Puk
  • Function : Author
  • PersonId : 947150
Michel Roux
David Adams
  • Function : Author
  • PersonId : 947151
Abdel Ayadi
  • Function : Author
  • PersonId : 947153
Lore Becker
  • Function : Author
  • PersonId : 947154
Marie-France Champy
  • Function : Author
  • PersonId : 947158
Roy Combe
  • Function : Author
  • PersonId : 947159
Elisabetta Golini
  • Function : Author
  • PersonId : 947164
Pierre Jurdic
  • Function : Author
  • PersonId : 947169
Frauke Neff
  • Function : Author
  • PersonId : 947173
Jan Rozman
  • Function : Author
  • PersonId : 947177
Mohammed Selloum
  • Function : Author
  • PersonId : 947180
Min Zi
  • Function : Author
  • PersonId : 947187
Karen Steel
  • Function : Author
  • PersonId : 947189
Yann Herault

Abstract

BACKGROUND: The mouse inbred line C57BL/6J is widely used in mouse genetics and its genome has been incorporated into many genetic reference populations. More recently large initiatives such as the International Knockout Mouse Consortium (IKMC) are using the C57BL/6N mouse strain to generate null alleles for all mouse genes. Hence both strains are now widely used in mouse genetics studies. Here we perform a comprehensive genomic and phenotypic analysis of the two strains to identify differences that may influence their underlying genetic mechanisms. RESULTS: We undertake genome sequence comparisons of C57BL/6J and C57BL/6N to identify SNPs, indels and structural variants, with a focus on identifying all coding variants. We annotate 34 SNPs and 2 indels that distinguish C57BL/6J and C57BL/6N coding sequences, as well as 15 structural variants that overlap a gene. In parallel we assess the comparative phenotypes of the two inbred lines utilizing the EMPReSSslim phenotyping pipeline, a broad based assessment encompassing diverse biological systems. We perform additional secondary phenotyping assessments to explore other phenotype domains and to elaborate phenotype differences identified in the primary assessment. We uncover significant phenotypic differences between the two lines, replicated across multiple centers, in a number of physiological, biochemical and behavioral systems. CONCLUSIONS: Comparison of C57BL/6J and C57BL/6N demonstrates a range of phenotypic differences that have the potential to impact upon penetrance and expressivity of mutational effects in these strains. Moreover, the sequence variants we identify provide a set of candidate genes for the phenotypic differences observed between the two strains.
Fichier principal
Vignette du fichier
gb-2013-14-7-r82.pdf (1.36 Mo) Télécharger le fichier
Vignette du fichier
GB-2013-14-7-R82-S1.PDF (225.09 Ko) Télécharger le fichier
Vignette du fichier
GB-2013-14-7-R82-S2.PDF (544.06 Ko) Télécharger le fichier
Vignette du fichier
GB-2013-14-7-R82-S3.PDF (2.92 Mo) Télécharger le fichier
Vignette du fichier
gb-2013-14-7-r82.xml (168.65 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Format : Other
Format : Other
Format : Other
Format : Other

Dates and versions

inserm-00874969 , version 1 (20-10-2013)

Identifiers

Cite

Michelle Simon, Simon Greenaway, Jacqueline White, Helmut Fuchs, Valérie Gailus-Durner, et al.. A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains.. Genome Biology, 2013, 14 (7), pp.R82. ⟨10.1186/gb-2013-14-7-r82⟩. ⟨inserm-00874969⟩
1141 View
638 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More