Inverse relationship between TCTP/RhoA and p53 /cyclin A/actin expression in ovarian cancer cells.

Malgorzata Kloc 1, 2 Neelam Tejpal 1, 2 Jitinderpal Sidhu 2 Malathesha Ganachari 2 Pedro Flores-Villanueva 2 Nicholas Jennings 3 Anil Sood 3 Jacek Kubiak 4, * Rafik Ghobrial 1, 2
* Auteur correspondant
1 Department of Surgery
2 Methodist Hospital Research Institute
3 Departments of Gynecologic Oncology and Cancer Biology
4 IGDR - Institut de Génétique et Développement de Rennes
Abstract : The translationally controlled tumor protein (TCTP) plays a role in cell growth, cell cycle and cancer progression. TCTP controls negatively the stability of the p53 tumor suppressor protein and interacts with the cellular cytoskeleton. The deregulation of the actin and cytokeratin cytoskeleton is responsible for the increased migratory activity of tumor cells and is linked with poor patient outcome. Recent studies indicate that cyclin A,a key regulator of cell cycle, controls actin organization and negatively regulates cell motility via regulation of RhoA expression. We studied the organization of actin and cytokeratin cytoskeleton and the expression of TCTP, p53,cyclin A, RhoA and actin in HIO180 non-transformed ovarian epithelial cells, and OVCAR3 and SKOV3 (expressing low level of inducible p53) ovarian epithelial cancer cells with different metastatic potential. Immunostaining and ultrastructural analyses illustrated a dramatic difference in the organization of the cytokeratin and actin filaments in non-transformed versus cancer cell lines. We also determined that there is an inverse relationship between the level of TCTP/RhoA and actin/p53/cyclin A expression in ovarian cancer cell lines. This previously unidentified negative relationship between TCTP/RhoA and actin/p53/cyclin A may suggest that this interaction is linked with the high aggressiveness of ovarian cancers.
Keywords : TCTP RhoA p53 cyclin A actin cytokeratin ovarian cancer cell lines
Type de document :
Article dans une revue
Folia Histochemica et Cytobiologica, Polskiego Towarzystwa Histochemików i Cytochemików (Polish Histochemical and Cytochemical Society), 2012, 50 (3), pp.358-67. 〈10.5603/19745〉
Liste complète des métadonnées

Littérature citée [48 références]  Voir  Masquer  Télécharger
http://www.hal.inserm.fr/inserm-00870561
Contributeur : Morgane Le Corre <>
Soumis le : lundi 7 octobre 2013 - 15:26:23
Dernière modification le : mercredi 16 mai 2018 - 11:23:52

Fichier

Inverse_relationship.accepted....
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

IGDR | BIOSIT | UR1-UFR-SVE | UNIV-RENNES

Citation

Malgorzata Kloc, Neelam Tejpal, Jitinderpal Sidhu, Malathesha Ganachari, Pedro Flores-Villanueva, et al.. Inverse relationship between TCTP/RhoA and p53 /cyclin A/actin expression in ovarian cancer cells.. Folia Histochemica et Cytobiologica, Polskiego Towarzystwa Histochemików i Cytochemików (Polish Histochemical and Cytochemical Society), 2012, 50 (3), pp.358-67. 〈10.5603/19745〉. 〈inserm-00870561〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

223

Téléchargements de fichiers

299

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD