Skip to Main content Skip to Navigation
Journal articles

An in vitro network of intermolecular interactions between viral RNA segments of an avian H5N2 influenza A virus: comparison with a human H3N2 virus.

Abstract : The genome of influenza A viruses (IAV) is split into eight viral RNAs (vRNAs) that are encapsidated as viral ribonucleoproteins. The existence of a segment-specific packaging mechanism is well established, but the molecular basis of this mechanism remains to be deciphered. Selective packaging could be mediated by direct interaction between the vRNA packaging regions, but such interactions have never been demonstrated in virions. Recently, we showed that the eight vRNAs of a human H3N2 IAV form a single interaction network in vitro that involves regions of the vRNAs known to contain packaging signals in the case of H1N1 IAV strains. Here, we show that the eight vRNAs of an avian H5N2 IAV also form a single network of interactions in vitro, but, interestingly, the interactions and the regions of the vRNAs they involve differ from those described for the human H3N2 virus. We identified the vRNA sequences involved in five of these interactions at the nucleotide level, and in two cases, we validated the existence of the interaction using compensatory mutations in the interacting sequences. Electron tomography also revealed significant differences in the interactions taking place between viral ribonucleoproteins in H5N2 and H3N2 virions, despite their canonical '7 + 1' arrangement.
Document type :
Journal articles
Complete list of metadatas

Cited literature [48 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00870461
Contributor : Morgane Le Corre <>
Submitted on : Monday, October 7, 2013 - 1:16:12 PM
Last modification on : Thursday, June 4, 2020 - 12:32:07 AM

File

An_in_vitro_network-accepted.p...
Publisher files allowed on an open archive

Identifiers

Citation

Cyrille Gavazzi, Catherine Isel, Emilie Fournier, Vincent Moules, Annie Cavalier, et al.. An in vitro network of intermolecular interactions between viral RNA segments of an avian H5N2 influenza A virus: comparison with a human H3N2 virus.. Nucleic Acids Research, Oxford University Press, 2013, 41 (2), pp.1241-54. ⟨10.1093/nar/gks1181⟩. ⟨inserm-00870461⟩

Share

Metrics

Record views

588

Files downloads

918