Regulatory CD4+CD25hi T cells conserve their function and phenotype after granulocyte colony-stimulating factor treatment in human hematopoietic stem cell transplantation.

Grégory Noël 1 Denis Bruniquel 2, 1 Sophie Deguibert 1, 3 Brigitte Birebent 1, 2 Jean-Marc Grosset 2 Marc Bernard 3 Charlie Dauriac 3 Thierry Lamy-De-La-Chapelle 3 Gilbert Semana 1, 2 Carine Brinster 1, 2, *
* Auteur correspondant
1 Laboratoire d'Immuno-Hématologie
2 Etablissement Français du Sang Bretagne
3 Service d'hématologie clinique
Abstract : Acute graft-versus-host disease (aGVHD), mediated by CD4(+) and CD8(+) effector T cells, is a life-threatening complication in hematopoietic stem cell transplantation. CD4(+)CD25(hi) regulatory T cells (T(reg)) have been shown to modulate tolerance to aGVHD in murine models. Based on these observations, we examined their role in the prevention of aGVHD in patients who underwent transplantation with peripheral blood-mobilized hematopoietic stem cells after administration of granulocyte colony-stimulating factor. The effects of the G-CSF on the phenotype, frequency, and function of CD4(+)CD25(hi) T cells were analyzed in grafts and after transplantation to determine whether these cells were regulatory T cells. CD4(+)CD25(hi) T cells could be detected at the same frequency before and after granulocyte colony-stimulating factor administration in the donors' peripheral blood. The isolation of these cells from the grafts or from the recipients' peripheral blood after transplantation revealed that they were suppressive to the same extent as T(reg) isolated from healthy volunteers. Their number and frequency were estimated in the grafts and the results indicated that protection against aGVHD was not dependent on the T(reg) amount transferred to the recipients. Similarly there was no correlation between the number of circulating CD4(+)CD25(hi) T cells in the recipients' peripheral blood during the early period after transplantation and the outcome of aGVHD.
Keywords : Regulatory T cells Tolerance Transplantation Granulocyte colony-stimulating factor Acute graft-versus-host disease
Type de document :
Article dans une revue
Human Immunology, Elsevier, 2008, 69 (6), pp.329-37. 〈10.1016/j.humimm.2008.04.008〉
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https://www.hal.inserm.fr/inserm-00869456
Contributeur : Morgane Le Corre <>
Soumis le : jeudi 3 octobre 2013 - 13:22:26
Dernière modification le : mercredi 16 mai 2018 - 11:23:18

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Grégory Noël, Denis Bruniquel, Sophie Deguibert, Brigitte Birebent, Jean-Marc Grosset, et al.. Regulatory CD4+CD25hi T cells conserve their function and phenotype after granulocyte colony-stimulating factor treatment in human hematopoietic stem cell transplantation.. Human Immunology, Elsevier, 2008, 69 (6), pp.329-37. 〈10.1016/j.humimm.2008.04.008〉. 〈inserm-00869456〉

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