ROQUIN/RC3H1 alterations are not found in angioimmunoblastic T-cell lymphoma. - Archive ouverte HAL Access content directly
Journal Articles PLoS ONE Year : 2013

ROQUIN/RC3H1 alterations are not found in angioimmunoblastic T-cell lymphoma.

(1) , (1) , (2) , (2) , (1, 3) , (1, 3) , (4) , (5) , (1, 3) , (1, 6)
1
2
3
4
5
6

Abstract

Angioimmunoblastic T-cell Lymphoma (AITL) is one of the most frequent T-cell lymphoma entities. Follicular helper T lymphocytes (TFH) are recognized as the normal cellular counterpart of the neoplastic component. Despite a clonal T-cell feature and few described recurrent cytogenetic abnormalities, a driving oncogenic event has not been identified so far. It has been recently reported that in mice, heterozygous inactivation of Roquin/Rc3h1, a RING type E3 ubiquitine ligase, recapitulates many of the clinical, histological, and cellular features associated with human AITL. In this study we explored whether ROQUIN alterations could be an initial event in the human AITL oncogenic process. Using microarray and RT-PCR analyses, we investigated the levels of ROQUIN transcripts in TFH tumor cells purified from AITL (n = 8) and reactive tonsils (n = 12) and found similar levels of ROQUIN expression in both. Moreover, we also demonstrated that ROQUIN protein was expressed by AITL TFH (PD1+) cells. We then analysed ROQUIN coding sequence in 12 tumor cell-rich AITL samples and found no mutation in any of the samples. Finally, we analysed the expression of MiR101, a putative partner of ROQUIN involved in the modulation of ICOS expression and found similar levels of expression in tumor and reactive TFH. Altogether, this study shows that neither alteration of ROQUIN gene nor deregulation of miR101 expression is likely to be a frequent recurrent event in AITL.
Fichier principal
Vignette du fichier
pone.0064536.pdf (429.69 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

inserm-00868981 , version 1 (02-10-2013)

Identifiers

Cite

Tiphanie Auguste, Marion Travert, Karin Tarte, Patricia Amé-Thomas, Catherine Artchounin, et al.. ROQUIN/RC3H1 alterations are not found in angioimmunoblastic T-cell lymphoma.. PLoS ONE, 2013, 8 (6), pp.e64536. ⟨10.1371/journal.pone.0064536⟩. ⟨inserm-00868981⟩
157 View
162 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More