 |
Journal articles
New tensio-active molecules stabilize a human G protein-coupled receptor in solution.
Abstract : Structural characterization of membrane proteins is hampered by the instability of the isolated proteins in detergent solutions. Here, we describe a new class of phospholipid-like surfactants that stabilize the G protein-coupled receptor, BLT1. These compounds, called C(13)U(9), C(13)U(19), C(15)U(25) and C(17)U(16), were synthesized by radical polymerization of Tris(hydroxymethyl) acrylamidomethane in the presence of thioglycerol, first endowed with two hydrocarbon chains with variable lengths (13-17 carbon atoms), as transfer reagent. C(13)U(19), C(17)U(16) or C(15)U(25) significantly enhanced the stability of BLT1 in solution compared to what was obtained with common detergents. These molecules therefore represent a promising step towards the structural characterization of BLT1 and possibly other membrane proteins.
Cited literature [28 references]
https://www.hal.inserm.fr/inserm-00868640
Contributor : Isabelle Arnal <>
Submitted on : Tuesday, October 1, 2013 - 4:50:08 PM
Last modification on : Wednesday, November 4, 2020 - 2:33:36 PM
Long-term archiving on: : Friday, April 7, 2017 - 4:43:35 AM
Contributor : Isabelle Arnal <>
Submitted on : Tuesday, October 1, 2013 - 4:50:08 PM
Last modification on : Wednesday, November 4, 2020 - 2:33:36 PM
Long-term archiving on: : Friday, April 7, 2017 - 4:43:35 AM