Identification of pVHL as a novel substrate for Aurora-A in clear cell renal cell carcinoma (ccRCC).

Benedicte Martin 1 Franck Chesnel 1 Jean-Guy Delcros 2 Florence Jouan 1 Anne Couturier 1 Frederic Dugay 1 Xavier Le Goff 1 Jean-Jacques Patard 3 Patricia Fergelot 4 Cecile Vigneau 1 Nathalie Rioux-Leclerq 1 Yannick Arlot-Bonnemains 1, *
* Auteur correspondant
1 Cancer du rein : bases moléculaires de la tumorogenèse
IGDR - Institut de Génétique et Développement de Rennes
2 CRCL - Centre de Recherche en Cancérologie de Lyon
3 Service de Génétique Moléculaire
4 Service de Chirurgie Urologie
Abstract : Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer and is often characterized by mutations or deletions of the Von Hippel Lindau (VHL) tumour suppressor gene. Aurora gene family members are implicated in proper mitotic progression and spindle checkpoint function and play a crucial role in cancer progression. In the present study, we assessed the expression of Aurora-A in a cohort of 30 ccRCC with fully characterized VHL status (wt/wt or mut/del) and Fuhrman grade. Aurora-A transcript and protein levels were significantly increased in high Fuhrman grade tumours and in VHLwt/wt tumours. These results suggest that Aurora-A and VHL interact in the ccRCC. We demonstrated that the two proteins interact in vivo and identified the Ser72 on the sequence of VHL as the unique site phosphorylated by Aurora-A.
Keywords : carcinogenesis cell cycle and cell division immunoprecipitation microtubules phosphorylation protein expression protein extraction tumor physiology
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2013, 8 (6), pp.e67071. 〈10.1371/journal.pone.0067071〉
Liste complète des métadonnées
http://www.hal.inserm.fr/inserm-00865341
Contributeur : Clémence Martin <>
Soumis le : mardi 24 septembre 2013 - 12:54:39
Dernière modification le : mercredi 16 mai 2018 - 11:24:09
Document(s) archivé(s) le : mercredi 25 décembre 2013 - 04:33:22

Fichier

PMID_23785518.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

IGDR | BIOSIT | NUMECAN | NUMECAN-CIMIAD | UR1-UFR-SVE | UNIV-LYON1 | UNIV-RENNES | CRCL

Citation

Benedicte Martin, Franck Chesnel, Jean-Guy Delcros, Florence Jouan, Anne Couturier, et al.. Identification of pVHL as a novel substrate for Aurora-A in clear cell renal cell carcinoma (ccRCC).. PLoS ONE, Public Library of Science, 2013, 8 (6), pp.e67071. 〈10.1371/journal.pone.0067071〉. 〈inserm-00865341〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

566

Téléchargements de fichiers

276

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD