Skip to Main content Skip to Navigation
Journal articles

Impact of Anesthetics on Immune Functions in a Rat Model of Vagus Nerve Stimulation.

Chloé Picq 1, * Didier Clarençon 1 Valérie Sinniger 2 Bruno Bonaz 3 Jean-François Mayol 4, 5
* Corresponding author
1 INSERM U836, équipe 8, Stress et interactions neurodigestives
CRSSA - Centre de Recherches du Service de Santé des Armées, GIN - Grenoble Institut des Neurosciences
2 INSERM U836, équipe 8, Stress et interactions neurodigestives
GIN - Grenoble Institut des Neurosciences
3 INSERM U836, équipe 8, Stress et interactions neurodigestives
GIN - Grenoble Institut des Neurosciences, Clinique Universitaire d'Hépato-Gastroentérologie
Abstract : : Vagus nerve stimulation (VNS) has been successfully performed in animals for the treatment of different experimental models of inflammation. The anti-inflammatory effect of VNS involves the release of acetylcholine by vagus nerve efferent fibers inhibiting pro-inflammatory cytokines (e.g. TNF-α) produced by macrophages. Moreover, it has recently been demonstrated that splenic lymphocytic populations may also be involved. As anesthetics can modulate the inflammatory response, the current study evaluated the effect of two different anesthetics, isoflurane and pentobarbital, on splenic cellular and molecular parameters in a VNS rat model. Spleens were collected for the characterization of lymphocytes sub-populations by flow cytometry and quantification of cytokines secretion after in vitro activation. Different results were observed depending on the anesthetic used. The use of isoflurane displayed a non-specific effect of VNS characterized by a decrease of most splenic lymphocytes sub-populations studied, and also led to a significantly lower TNF-α secretion by splenocytes. However, the use of pentobarbital brought to light immune modifications in non-stimulated animals that were not observed with isoflurane, and also revealed a specific effect of VNS, notably at the level of T lymphocytes' activation. These differences between the two anesthetics could be related to the anti-inflammatory properties of isoflurane. In conclusion, pentobarbital is more adapted than isoflurane in the study of the anti-inflammatory effect of VNS on an anesthetized rat model in that it allows more accurate monitoring of subtle immunomodulatory processes.
Complete list of metadatas

Cited literature [31 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00859188
Contributor : Valérie Sinniger <>
Submitted on : Friday, September 6, 2013 - 4:11:07 PM
Last modification on : Thursday, July 11, 2019 - 2:10:07 PM
Long-term archiving on: : Thursday, April 6, 2017 - 4:21:19 PM

File

Picq-2013_Impact_AO.pdf
Publisher files allowed on an open archive

Identifiers

Collections

UGA | SSA | U836

Citation

Chloé Picq, Didier Clarençon, Valérie Sinniger, Bruno Bonaz, Jean-François Mayol. Impact of Anesthetics on Immune Functions in a Rat Model of Vagus Nerve Stimulation.. PLoS ONE, Public Library of Science, 2013, 8 (6), pp.e67086. ⟨10.1371/journal.pone.0067086⟩. ⟨inserm-00859188⟩

Share

Metrics

Record views

455

Files downloads

739