Effect of particle size on the biodistribution of lipid nanocapsules: Comparison between nuclear and fluorescence imaging and counting.

Samuli Hirsjärvi 1, * Lucie Sancey 2, 3 Sandrine Dufort 2 Camille Belloche 1 Claire Vanpouille-Box 1 Emmanuel Garcion 1 Jean-Luc Coll 2 François Hindré 1 Jean-Pierre Benoit 1, *
* Auteur correspondant
1 MINT - Micro et nanomédecines biomimétiques
2 INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble
3 ILM - Institut Lumière Matière [Villeurbanne]
Abstract : In vivo biodistribution of nanoparticles depends on several physicochemical parameters such as size. After intravenous injection of 25, 50 and 100nm lipid nanocapsules (LNC) in nude mice bearing HEK293(β3) tumour xenografts, biodistribution was evaluated by γ-scintigraphy and by γ-counting. The small LNC 25nm disappeared faster than the larger LNC 50 and 100nm from the blood circulation due to faster elimination and wider tissue distribution. At 24h, biodistribution profiles of all these LNC were similar. Low LNC quantities were found in this weak EPR (enhanced permeability and retention) tumour regardless the particle size. Co-injected 50nm fluorescent DiD-LNC and (99m)Tc-LNC allowed direct comparison of biodistribution as evaluated by the two methods. Optical imaging underestimated LNC quantity especially in dark-colored organs that were observed to capture extensive quantities of the particles by γ-counting (i.e. liver, spleen, and kidney).
Keywords : lipid nanocapsules biodistribution γ-scintigraphy optical imaging fluorescence
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Article dans une revue
International Journal of Pharmaceutics, Elsevier, 2013, 453 (2), pp.594-600. 〈10.1016/j.ijpharm.2013.05.057〉
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Samuli Hirsjärvi, Lucie Sancey, Sandrine Dufort, Camille Belloche, Claire Vanpouille-Box, et al.. Effect of particle size on the biodistribution of lipid nanocapsules: Comparison between nuclear and fluorescence imaging and counting.. International Journal of Pharmaceutics, Elsevier, 2013, 453 (2), pp.594-600. 〈10.1016/j.ijpharm.2013.05.057〉. 〈inserm-00855672〉

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