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Journal articles
Adipocyte-Derived Fibroblasts promote tumor progression and contribute to desmoplastic reaction in breast cancer.
Ludivine Bochet
1, 2
Camille Lehuede
1, 2
Stephanie Dauvillier
2
Yuan Yuan Wang
1, 2
Beatrice Dirat
1, 2
Victor Laurent
1
Cédric Dray
2
Romain Guiet
1
Isabelle Maridonneau-Parini
1
Sophie Le Gonidec
2
Bettina Couderc
3
Ghislaine Escourrou
4
Philippe Valet
2
Catherine Muller
1, 2, *
Ludivine Bochet 1, 2
Author
Camille Lehuede 1, 2
Author
Stephanie Dauvillier 2
Author
Yuan Yuan Wang 1, 2
Author
Isabelle Maridonneau-Parini 1
Author
Sophie Le Gonidec 2
Author
Bettina Couderc 3
Author
Ghislaine Escourrou 4
Author
Philippe Valet 2
Author
Catherine Muller 1, 2
Correspondent author
1
IPBS - Institut de pharmacologie et de biologie structurale (205 Route de Narbonne 31077 TOULOUSE CEDEX 4 - France)
- UT3 - Université Toulouse III - Paul Sabatier (118 route de Narbonne - 31062 Toulouse - France)
- Université Fédérale Toulouse Midi-Pyrénées (41 Allée Jules Guesde, 31000 Toulouse - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5089 (France)
2
I2MC - Institut des Maladies Métaboliques et Cardiovasculaires (1 avenue du Prof Jean Poulhes - BP 84225 - 31432 Toulouse Cedex 4 - France)
- UT3 - Université Toulouse III - Paul Sabatier (118 route de Narbonne - 31062 Toulouse - France)
- Université Fédérale Toulouse Midi-Pyrénées (41 Allée Jules Guesde, 31000 Toulouse - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : UMR1048 (101, rue de Tolbiac, 75013 Paris - France)
3
EA3035 (Groupe de pharmacologie Clinique et expérimentale des médicaments anticancéreux, 20-24 rue du Pont Saint-Pierre 31052 Toulouse Cedex - France)
- Institut Claudius Regaud (France)
- CLCC Toulouse (France)
4
Service d'Anatomo-Pathologie et Histologie-Cytologie (Pôle Biologie, 1 avenue du Professeur Jean Poulhès TSA 50032 31059 Toulouse cedex 9 - France)
- CHU Toulouse [Toulouse] (Hôtel-Dieu Saint Jacques 2, rue Viguerie TSA 80035 31059 Toulouse cedex 9 - France)
- Hôpital de Rangueil (France)
- CHU Toulouse [Toulouse] (Hôtel-Dieu Saint Jacques 2, rue Viguerie TSA 80035 31059 Toulouse cedex 9 - France)
Abstract : Cancer-associated fibroblasts (CAFs) comprise the majority of stromal cells in breast cancers, yet their precise origins and relative functional contributions to malignant progression remain uncertain. Local invasion leads to the proximity cancer cells and adipocytes, which respond by phenotypical changes to generate fibroblast-like cells termed here adipocyte-derived fibroblasts (ADFs). These cells exhibit enhanced secretion of fibronectin and collagen I, increased migratory/invasive abilities and increased expression of the CAF marker FSP-1 but not αSMA. Generation of the ADF phenotype depends on reactivation of the Wnt/β-catenin pathway in response to Wnt3a secreted by tumor cells. Tumor cells co-cultivated with ADFs in 2D or spheroid culture display increased invasive capabilities. In clinical specimens of breast cancer, we confirmed the presence of this new stromal sub-population. By defining a new stromal cell population, our results offer new opportunities for stroma-targeted therapies in breast cancer.
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https://www.hal.inserm.fr/inserm-00852355
Contributor : Marie Francoise Simon <>
Submitted on : Tuesday, August 20, 2013 - 4:43:21 PM
Last modification on : Friday, October 16, 2020 - 10:14:14 AM
Contributor : Marie Francoise Simon <>
Submitted on : Tuesday, August 20, 2013 - 4:43:21 PM
Last modification on : Friday, October 16, 2020 - 10:14:14 AM
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- HAL Id : inserm-00852355, version 1
- DOI : 10.1158/0008-5472.CAN-13-0530
- PUBMED : 23903958
Collections
INSERM | CNRS | UNIV-TLSE3 | IPBS
Citation
Ludivine Bochet, Camille Lehuede, Stephanie Dauvillier, Yuan Yuan Wang, Beatrice Dirat, et al.. Adipocyte-Derived Fibroblasts promote tumor progression and contribute to desmoplastic reaction in breast cancer.. Cancer Research, American Association for Cancer Research, 2013, epub ahead of print. ⟨10.1158/0008-5472.CAN-13-0530⟩. ⟨inserm-00852355⟩
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