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Adipose-derived stromal cells: cytokine expression and immune cell contaminants.

Abstract : The present method describes an immunoselection/depletion approach to isolate the native human adipose tissue-derived progenitor cells that are free from endothelial cells and immune cells by the use of magnetic nanobeads and microbeads coupled to antibodies. Moreover, methods to isolate and to analyse the distinct cell populations that constitute the microenvironment of the human adipose tissue progenitor cells, i.e. mature adipocytes, endothelial cells, and macrophages, are mentioned.
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https://www.hal.inserm.fr/inserm-00852298
Contributor : Marie Francoise Simon <>
Submitted on : Tuesday, August 20, 2013 - 3:23:50 PM
Last modification on : Friday, January 10, 2020 - 9:09:54 PM

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Pauline Decaunes, David Estève, Alexia Zakaroff-Girard, Coralie Sengenès, Jean Galitzky, et al.. Adipose-derived stromal cells: cytokine expression and immune cell contaminants.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2011, 702, pp.151-61. ⟨10.1007/978-1-61737-960-4_12⟩. ⟨inserm-00852298⟩

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