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Journal Articles Methods in Molecular Biology Year : 2011

Adipose-derived stromal cells: cytokine expression and immune cell contaminants.

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Abstract

The present method describes an immunoselection/depletion approach to isolate the native human adipose tissue-derived progenitor cells that are free from endothelial cells and immune cells by the use of magnetic nanobeads and microbeads coupled to antibodies. Moreover, methods to isolate and to analyse the distinct cell populations that constitute the microenvironment of the human adipose tissue progenitor cells, i.e. mature adipocytes, endothelial cells, and macrophages, are mentioned.
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inserm-00852298 , version 1 (20-08-2013)

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Pauline Decaunes, David Estève, Alexia Zakaroff-Girard, Coralie Sengenès, Jean Galitzky, et al.. Adipose-derived stromal cells: cytokine expression and immune cell contaminants.. Methods in Molecular Biology, 2011, 702, pp.151-61. ⟨10.1007/978-1-61737-960-4_12⟩. ⟨inserm-00852298⟩
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