Skip to Main content Skip to Navigation
Journal articles

A novel monoclonal anti-CD81 antibody produced by genetic immunization efficiently inhibits Hepatitis C virus cell-cell transmission.

Abstract : BACKGROUND AND AIMS: Hepatitis C virus (HCV) infection is a challenge to prevent and treat because of the rapid development of drug resistance and escape. Viral entry is required for initiation, spread, and maintenance of infection, making it an attractive target for antiviral strategies. METHODS: Using genetic immunization, we produced four monoclonal antibodies (mAbs) against the HCV host entry factor CD81. The effects of antibodies on inhibition of HCV infection and dissemination were analyzed in HCV permissive human liver cell lines. RESULTS: The anti-CD81 mAbs efficiently inhibited infection by HCV of different genotypes as well as a HCV escape variant selected during liver transplantation and re-infecting the liver graft. Kinetic studies indicated that anti-CD81 mAbs target a post-binding step during HCV entry. In addition to inhibiting cell-free HCV infection, one antibody was also able to block neutralizing antibody-resistant HCV cell-cell transmission and viral dissemination without displaying any detectable toxicity. CONCLUSION: A novel anti-CD81 mAb generated by genetic immunization efficiently blocks HCV spread and dissemination. This antibody will be useful to further unravel the role of virus-host interactions during HCV entry and cell-cell transmission. Furthermore, this antibody may be of interest for the development of antivirals for prevention and treatment of HCV infection.
Complete list of metadata

Cited literature [53 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00850895
Contributor : Thomas F. Baumert Connect in order to contact the contributor
Submitted on : Friday, August 9, 2013 - 2:20:41 PM
Last modification on : Monday, December 14, 2020 - 4:30:32 PM
Long-term archiving on: : Sunday, November 10, 2013 - 4:17:54 AM

File

journal.pone.0064221.pdf
Publisher files allowed on an open archive

Identifiers

Collections

`

Citation

Isabel Fofana, Fei Xiao, Christine Thumann, Marine Turek, Laetitia Zona, et al.. A novel monoclonal anti-CD81 antibody produced by genetic immunization efficiently inhibits Hepatitis C virus cell-cell transmission.. PLoS ONE, Public Library of Science, 2013, 8 (5), pp.e64221. ⟨10.1371/journal.pone.0064221⟩. ⟨inserm-00850895⟩

Share

Metrics

Record views

322

Files downloads

496