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Functional impact of Aurora A-mediated phosphorylation of HP1gamma at serine 83 during cell cycle progression.

Abstract : BACKGROUND: Previous elegant studies performed in the fission yeast Schizosaccharomyces pombe have identified a requirement for heterochromatin protein 1 (HP1) for spindle pole formation and appropriate cell division. In mammalian cells, HP1gamma has been implicated in both somatic and germ cell proliferation. High levels of HP1gamma protein associate with enhanced cell proliferation and oncogenesis, while its genetic inactivation results in meiotic and mitotic failure. However, the regulation of HP1gamma by kinases, critical for supporting mitotic progression, remains to be fully characterized. RESULTS: We report for the first time that during mitotic cell division, HP1gamma colocalizes and is phosphorylated at serine 83 (Ser83) in G2/M phase by Aurora A. Since Aurora A regulates both cell proliferation and mitotic aberrations, we evaluated the role of HP1gamma in the regulation of these phenomena using siRNA-mediated knockdown, as well as phosphomimetic and nonphosphorylatable site-directed mutants. We found that genetic downregulation of HP1gamma, which decreases the levels of phosphorylation of HP1gamma at Ser83 (P-Ser83-HP1gamma), results in mitotic aberrations that can be rescued by reintroducing wild type HP1gamma, but not the nonphosphorylatable S83A-HP1gamma mutant. In addition, proliferation assays showed that the phosphomimetic S83D-HP1gamma increases 5-ethynyl-2 -deoxyuridine (EdU) incorporation, whereas the nonphosphorylatable S83A-HP1gamma mutant abrogates this effect. Genome-wide expression profiling revealed that the effects of these mutants on mitotic functions are congruently reflected in G2/M gene expression networks in a manner that mimics the on and off states for P-Ser83-HP1gamma. CONCLUSIONS: This is the first description of a mitotic Aurora A-HP1gamma pathway, whose integrity is necessary for the execution of proper somatic cell division, providing insight into specific types of posttranslational modifications that associate to distinct functional outcomes of this important chromatin protein.
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Submitted on : Wednesday, July 10, 2013 - 1:09:57 PM
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Adrienne Grzenda, Phoebe Leonard, Seungmae Seo, Angela Mathison, Guillermo Urrutia, et al.. Functional impact of Aurora A-mediated phosphorylation of HP1gamma at serine 83 during cell cycle progression.. Epigenetics and Chromatin, BioMed Central, 2013, 6 (1), pp.21. ⟨10.1186/1756-8935-6-21⟩. ⟨inserm-00843064⟩



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