Native Adipose Stromal Cells (ASCs) Egress from Adipose Tissue in vivo: Evidence During Lymph Node Activation. - Archive ouverte HAL Access content directly
Journal Articles Stem Cells / Stem Cells (Miamisburg) Year : 2013

Native Adipose Stromal Cells (ASCs) Egress from Adipose Tissue in vivo: Evidence During Lymph Node Activation.

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Abstract

Adipose tissue (AT) has become accepted as a source of multipotent progenitor cells, the adipose derived stroma/stem cells (ASCs). In this regard, considerable work has been performed to harvest and characterize this cell population as well as to investigate the mechanisms by which transplanted ASCs mediate tissue regeneration. In contrast the endogenous release of native ASCs by AT has been poorly investigated. In the present work, we show that native ASCs egress from murine AT. Indeed, we demonstrated that the release of native ASCs from AT can be evidenced both using an ex vivo perfusion model that we set-up and in vivo. Such a mobilization process is controlled by CXCR4 chemokine receptor. In addition, once mobilized from AT, circulating ASCs were found to navigate through lymph fluid and to home into lymph nodes (LN). Therefore we demonstrated that, during the LN activation, the fat depot encapsulating the activated LN releases native ASCs, which in turn invade the activated LN. Moreover, the ASCs invading the LN were vizualized in close physical interaction with podoplanin and ER-TR7 positive structures corresponding to the stromal network composing the LN. This dynamic was impaired with CXCR4 neutralizing antibody. Taken together, these data provide robust evidences that native ASCs can traffic in vivo and that AT might provide stromal cells to activated LNs.

Dates and versions

inserm-00840905 , version 1 (03-07-2013)

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Cite

Marta Gil-Ortega, Lucile Garidou, Corinne Barreau, Marie Maumus, Ludovic Breasson, et al.. Native Adipose Stromal Cells (ASCs) Egress from Adipose Tissue in vivo: Evidence During Lymph Node Activation.. Stem Cells / Stem Cells (Miamisburg), 2013, epub ahead of print. ⟨10.1002/stem.1375⟩. ⟨inserm-00840905⟩
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