Role of warm ischemia on innate and adaptive responses in a preclinical renal auto-transplanted porcine model. - Archive ouverte HAL Access content directly
Journal Articles Journal of Translational Medicine Year : 2013

Role of warm ischemia on innate and adaptive responses in a preclinical renal auto-transplanted porcine model.

(1) , (1, 2, 3) , (1, 2) , (1, 2) , (1, 3) , (1, 2, 3) , (1) , (1, 2, 3, 4)
1
2
3
4

Abstract

BACKGROUND: Deceased after cardiac arrest donor are an additional source of kidney graft to overcome graft shortage. Deciphering the respective role of renal warm and cold ischemia is of pivotal interest in the transplantation process. METHODS: Using a preclinical pig model of renal auto-transplantation, we investigated the consequences of warm and cold ischemia on early innate and adaptive responses as well as graft outcome. Kidneys were subjected to either 60 min-warm ischemia (WI) or auto-transplanted after cold storage for 24 h at 4[degree sign]C (CS), or both conditions combined (WI + CS). Renal function, immune response and cytokine expression, oxidative stress and cell death were investigated at 3 h, 3 and 7 days (H3, D3 and D7) after reperfusion. At 3 months, we focused on cell infiltration and tissue remodelling. RESULTS: WI + CS induced a delayed graft function linked to higher tubular damage. Innate response occurred at D3 associated to a pro-oxidative milieu with a level dependent on the severity of ischemic injury whereas adaptive immune response occurred only at D7 mainly due to CS injuries and aggravated by WI. Graft cellular death was an early event detected at H3 and seems to be one of the first ischemia reperfusion injuries. These early injuries affect graft outcome on renal function, cells infiltration and fibrosis development. CONCLUSIONS: The results indicate that the severe ischemic insult found in kidneys from deceased after cardiac arrest donor affects kidney outcome and promotes an uncontrolled deleterious innate and adaptive response not inhibited 3 months after reperfusion.
Fichier principal
Vignette du fichier
1479-5876-11-129.pdf (839.57 Ko) Télécharger le fichier
Vignette du fichier
1479-5876-11-129-S1.PPT (123 Ko) Télécharger le fichier
Vignette du fichier
1479-5876-11-129.xml (70.15 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Format : Other
Format : Other
Loading...

Dates and versions

inserm-00827220 , version 1 (29-05-2013)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Ludivine Rossard, Frédéric Favreau, Sebastien Giraud, Raphael Thuillier, Sylvain Le Pape, et al.. Role of warm ischemia on innate and adaptive responses in a preclinical renal auto-transplanted porcine model.. Journal of Translational Medicine, 2013, 11 (1), pp.129. ⟨10.1186/1479-5876-11-129⟩. ⟨inserm-00827220⟩
523 View
199 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More