Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes.

Abstract : BACKGROUND: miRNA profiling performed in myogenic cells and biopsies from skeletal muscles has previously identified miRNAs involved in myogenesis. RESULTS: Here, we have performed miRNA transcriptome profiling in human affinity-purified CD56+ myoblasts induced to differentiate in vitro. In total, we have identified 60 miRNAs differentially expressed during myogenic differentiation. Many were not known for being differentially expressed during myogenic differentiation. Of these, 14 (miR-23b, miR-28, miR-98, miR-103, miR-107, miR-193a, miR-210, miR-324-5p, miR-324-3p, miR-331, miR-374, miR-432, miR-502, and miR-660) were upregulated and 6 (miR-31, miR-451, miR-452, miR-565, miR-594 and miR-659) were downregulated. mRNA transcriptome profiling performed in parallel resulted in identification of 6,616 genes differentially expressed during myogenic differentiation. CONCLUSIONS: This simultaneous miRNA/mRNA transcriptome profiling allowed us to predict with high accuracy target genes of myogenesis-related microRNAs and to deduce their functions.
Type de document :
Article dans une revue
BMC Genomics, BioMed Central, 2013, 14 (1), pp.265. 〈10.1186/1471-2164-14-265〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00819393
Contributeur : Ed. Bmc <>
Soumis le : mercredi 1 mai 2013 - 01:06:42
Dernière modification le : mercredi 4 juillet 2018 - 16:52:02

Identifiants

Collections

Citation

Petr Dmitriev, Ana Barat, Anna Polesskaya, Mary O'Connell, Thomas Robert, et al.. Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes.. BMC Genomics, BioMed Central, 2013, 14 (1), pp.265. 〈10.1186/1471-2164-14-265〉. 〈inserm-00819393〉

Partager

Métriques

Consultations de la notice

434

Téléchargements de fichiers

565