Inactivation of the LysR regulator Cj1000 of Campylobacter jejuni affects host colonization and respiration.

Virginie Dufour 1 Jennifer Li 2 Annika Flint 2 Eric Rosenfeld 3 Katell Rivoal 4 Sylvie Georgeault 1 Bachar Alazzam 1 Gwennola Ermel 1, 2 Alain Stintzi 2 Martine Bonnaure-Mallet 2 Christine Baysse 1, 2
1 ICM - Interactions cellulaires et moléculaires
2 Microbiologie : Risques Infectieux
3 LIENSs - LIttoral ENvironnement et Sociétés - UMR 7266
4 Laboratoire d'études et de recherches avicoles, porcines et piscicoles
Abstract : Transcriptional regulation mediates adaptation of pathogens to environmental stimuli and is important for host colonization. The Campylobacter jejuni genome sequence reveals a surprisingly small set of regulators, mostly of unknown function, suggesting an intricate regulatory network. Interestingly, C. jejuni lacks the homologues of ubiquitous regulators involved in stress response found in many other Gram-negative bacteria. Nonetheless, cj1000 is predicted to encode the sole LysR-type regulator in the C. jejuni genome, and thus may be involved in major adaptation pathways. A cj1000 mutant strain was constructed and found to be attenuated in its ability to colonize 1-day-old chicks. Complementation of the cj1000 mutation restored the colonization ability to wild-type levels. The mutant strain was also outcompeted in a competitive colonization assay of the piglet intestine. Oxygraphy was carried out for what is believed to be the first time with the Oroboros Oxygraph-2k on C. jejuni and revealed a role for Cj1000 in controlling O2 consumption. Furthermore, microarray analysis of the cj1000 mutant revealed both direct and indirect regulatory targets, including genes involved in energy metabolism and oxidative stress defences. These results highlight the importance of Cj1000 regulation in host colonization and in major physiological pathways.
Document type :
Journal articles
Complete list of metadatas
https://www.hal.inserm.fr/inserm-00816634
Contributor : Céline Allaire <>
Submitted on : Monday, April 22, 2013 - 4:04:23 PM
Last modification on : Tuesday, October 23, 2018 - 9:54:02 PM

Links full text

openaccess

Identifiers

Collections

ANSES | IGDR | UNIV-RENNES1 | BIOSIT | NUMECAN | NUMECAN-CIMIAD | UR1-UFR-SVE | UNIV-ROCHELLE | UNIV-RENNES

Citation

Virginie Dufour, Jennifer Li, Annika Flint, Eric Rosenfeld, Katell Rivoal, et al.. Inactivation of the LysR regulator Cj1000 of Campylobacter jejuni affects host colonization and respiration.. Microbiology, Microbiology Society, 2013, 159 (Pt 6), pp.1165-78. ⟨10.1099/mic.0.062992-0⟩. ⟨inserm-00816634⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

567

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD