Role of a short tandem leucine/arginine repeat in strong mutator phenotype acquisition in a clinical isolate of Salmonella Typhimurium. - Archive ouverte HAL Access content directly
Journal Articles FEMS Microbiology Letters Year : 2013

Role of a short tandem leucine/arginine repeat in strong mutator phenotype acquisition in a clinical isolate of Salmonella Typhimurium.

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Abstract

In this prospective study, a strong mutator strain of Salmonella Typhimurium was isolated from a collection of 130 human clinical strains of Salmonella. Sequence analysis of the mutS, mutL, and mutH genes, which encode three proteins that are essential for initiation of methyl-directed DNA mismatch repair, revealed insertion of a short tandem repeat (STR) of leucine/alanine in the histidine kinase-like ATPase domain of MutL. The role of this STR in the acquisition of the strong mutator phenotype was confirmed by the construction of an isogenic mutant (6bpinsmutL) from a normomutator strain of Salmonella Heidelberg. This result adds to the sparse body of knowledge about strong mutators and highlights the role of this STR as a hotspot for the acquisition of a strong mutator phenotype in Salmonella.

Dates and versions

inserm-00814119 , version 1 (16-04-2013)

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Hervé Le Bars, Latifa Bousarghin, Martine Bonnaure-Mallet, Anne Jolivet-Gougeon. Role of a short tandem leucine/arginine repeat in strong mutator phenotype acquisition in a clinical isolate of Salmonella Typhimurium.. FEMS Microbiology Letters, 2013, 338 (1), pp.101-6. ⟨10.1111/1574-6968.12039⟩. ⟨inserm-00814119⟩
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