Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies). - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles RNA Biology Year : 2013

Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies).

Abstract

A finely tuned balance of translation, storage and decay of mRNAs (mRNAs) is important for the regulation of gene expression. In eukaryotic cells, this takes place in dynamic cytoplasmic RNA-protein granules termed Processing bodies (P-bodies). In this study, by using immunoelectron tomography, 3D modeling and template matching, we analyze the size and the organization of the polysomes in the vicinity of human P-bodies. Our results show the presence of several polysomes that are compatible with a translational activity around P-bodies. Therefore, movement of mRNAs between polysomes and P-bodies can take place when the two compartments are in close contact. The presence of initiation factors in the proximity of P-bodies also suggests that translation of mRNAs can resume at the periphery of these granules.

Domains

Genetics

Dates and versions

inserm-00813732 , version 1 (16-04-2013)

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Cite

Nicolas Cougot, Anne-Elisabeth Molza, Emmanuel Giudice, Annie Cavalier, Daniel Thomas, et al.. Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies).. RNA Biology, 2013, 10 (2), pp.314-20. ⟨10.4161/rna.23342⟩. ⟨inserm-00813732⟩
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