Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies).

Nicolas Cougot 1 Anne-Elisabeth Molza 1 Emmanuel Giudice 1 Annie Cavalier 1 Daniel Thomas 1 Reynald Gillet 1
1 IGDR - Institut de Génétique et Développement de Rennes
Abstract : A finely tuned balance of translation, storage and decay of mRNAs (mRNAs) is important for the regulation of gene expression. In eukaryotic cells, this takes place in dynamic cytoplasmic RNA-protein granules termed Processing bodies (P-bodies). In this study, by using immunoelectron tomography, 3D modeling and template matching, we analyze the size and the organization of the polysomes in the vicinity of human P-bodies. Our results show the presence of several polysomes that are compatible with a translational activity around P-bodies. Therefore, movement of mRNAs between polysomes and P-bodies can take place when the two compartments are in close contact. The presence of initiation factors in the proximity of P-bodies also suggests that translation of mRNAs can resume at the periphery of these granules.
Keywords : P-bodies electron tomography initiation mRNA polysomes ribosomes
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Article dans une revue
RNA Biology, Taylor & Francis, 2013, 10 (2), pp.314-20. 〈10.4161/rna.23342〉
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http://www.hal.inserm.fr/inserm-00813732
Contributeur : Véronique Touya <>
Soumis le : mardi 16 avril 2013 - 10:52:26
Dernière modification le : mercredi 16 mai 2018 - 11:23:52

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IGDR | BIOSIT | UR1-UFR-SVE | UNIV-RENNES

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Nicolas Cougot, Anne-Elisabeth Molza, Emmanuel Giudice, Annie Cavalier, Daniel Thomas, et al.. Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies).. RNA Biology, Taylor & Francis, 2013, 10 (2), pp.314-20. 〈10.4161/rna.23342〉. 〈inserm-00813732〉

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