Th17 cytokines induce pro-fibrotic cytokines release from human eosinophils - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Respiratory Research Year : 2013

Th17 cytokines induce pro-fibrotic cytokines release from human eosinophils

Saleh Al-Muhsen
  • Function : Author
  • PersonId : 938246
Alejandro Vazquez-Tello
  • Function : Author
  • PersonId : 938248
Mary Angeline Pureza
  • Function : Author
  • PersonId : 938249
Ahmed Bahammam
  • Function : Author
  • PersonId : 938251
Qutayba Hamid
  • Function : Author
  • PersonId : 938252
Rabih Halwani
  • Function : Correspondent author
  • PersonId : 938253

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Subepithelial fibrosis is one of the most critical structural changes affecting bronchial airway function during asthma. Eosinophils have been shown to contribute to the production of pro-fibrotic cytokines, TGF-β and IL-11, however, the mechanism regulating this process is not fully understood.
In this report, we investigated whether cytokines associated with inflammation during asthma may induce eosinophils to produce pro-fibrotic cytokines.
Eosinophils were isolated from peripheral blood of 10 asthmatics and 10 normal control subjects. Eosinophils were stimulated with Th1, Th2 and Th17 cytokines and the production of TGF-β and IL-11 was determined using real time PCR and ELISA assays.
The basal expression levels of eosinophil derived TGF-β and IL-11 cytokines were comparable between asthmatic and healthy individuals. Stimulating eosinophils with Th1 and Th2 cytokines did not induce expression of pro-fibrotic cytokines. However, stimulating eosinophils with Th17 cytokines resulted in the enhancement of TGF-β and IL-11 expression in asthmatic but not healthy individuals. This effect of IL-17 on eosinophils was dependent on p38 MAPK activation as inhibiting the phosphorylation of p38 MAPK, but not other kinases, inhibited IL-17 induced pro-fibrotic cytokine release.
Th17 cytokines might contribute to airway fibrosis during asthma by enhancing production of eosinophil derived pro-fibrotic cytokines. Preventing the release of pro-fibrotic cytokines by blocking the effect of Th17 cytokines on eosinophils may prove to be beneficial in controlling fibrosis for disorders with IL-17 driven inflammation such as allergic and autoimmune diseases.
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inserm-00802220 , version 1 (19-03-2013)


  • HAL Id : inserm-00802220 , version 1


Saleh Al-Muhsen, Severine Letuve, Alejandro Vazquez-Tello, Mary Angeline Pureza, Hamdan Al-Jahdali, et al.. Th17 cytokines induce pro-fibrotic cytokines release from human eosinophils. Respiratory Research, 2013, 14 (1), pp.34. ⟨inserm-00802220⟩
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