Interferon block to HIV-1 transduction in macrophages despite SAMHD1 degradation and high deoxynucleoside triphosphates supply - Archive ouverte HAL Access content directly
Journal Articles Retrovirology Year : 2013

Interferon block to HIV-1 transduction in macrophages despite SAMHD1 degradation and high deoxynucleoside triphosphates supply

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Loic Dragin
  • Function : Author
  • PersonId : 938089
Laura Anh Nguyen
  • Function : Author
  • PersonId : 938090
Hichem Lahouassa
  • Function : Author
  • PersonId : 938091
Adèle Sourisce
  • Function : Author
  • PersonId : 938092
Baek Kim
  • Function : Author
  • PersonId : 938093
Bertha Cecilia Ramirez
  • Function : Author
  • PersonId : 938094
Florence Margottin-Goguet
  • Function : Correspondent author
  • PersonId : 918971

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Abstract

Background
Interferon-α (IFN-α) is an essential mediator of the antiviral response, which potently inhibits both early and late phases of HIV replication. The SAMHD1 deoxynucleoside triphosphate (dNTP) hydrolase represents the prototype of a new antiviral strategy we referred to as "nucleotide depletion". SAMHD1 depletes dNTP levels in myeloid cells below those required for optimal synthesis of HIV viral DNA. HIV-2 and its SIVsm and SIVmac close relatives encode a protein termed Vpx, which counteracts SAMHD1. The potentiality of IFN-α to cooperate with nucleotide depletion has been poorly investigated so far. Here we wondered whether IFN-α affects SAMHD1 expression, Vpx-induced SAMHD1 degradation, Vpx-mediated rescue of HIV-1 transduction and the dNTP supply in monocyte-derived macrophages (MDMs).
Results
IFN-α inhibited HIV-1 transduction in monocytes and in MDMs while SAMHD1 expression was not up-regulated. Vpx triggered SAMHD1 degradation in IFN-α treated cells, and weakly restored HIV-1 transduction from the IFN-α block. Vpx helper effect towards HIV-1 transduction was gradually inhibited with increasing doses of IFN-α. dNTP levels were not significantly affected in MDMs and CD4+ primary activated T lymphocytes by IFN-α and, in correlation with SAMHD1 degradation, restoration of dNTP levels by Vpx was efficient in MDMs treated with the cytokine. In contrast, IFN-α inhibited Vpx-mediated SAMHD1 degradation in THP-1 cells, where, accordingly, Vpx could not rescue HIV-1 transduction.
Conclusion
Our results suggest that the early antiviral effect of IFN-α results from a mechanism independent of nucleotide depletion in MDMs. In addition, they indicate that the macrophage-like THP-1 cell line may provide a system to characterize an IFN-α-induced cell response that inhibits Vpx-mediated SAMHD1 degradation.
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Dates and versions

inserm-00800768 , version 1 (14-03-2013)

Identifiers

  • HAL Id : inserm-00800768 , version 1

Cite

Loic Dragin, Laura Anh Nguyen, Hichem Lahouassa, Adèle Sourisce, Baek Kim, et al.. Interferon block to HIV-1 transduction in macrophages despite SAMHD1 degradation and high deoxynucleoside triphosphates supply. Retrovirology, 2013, 10 (1), pp.30. ⟨inserm-00800768⟩
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