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Journal articles
T Lymphocytes Orient against the Direction of Fluid Flow during LFA-1-Mediated Migration.
Abstract : As they leave the blood stream and travel to lymph nodes or sites of inflammation, T lymphocytes are captured by the endothelium and migrate along the vascular wall to permissive sites of transmigration. These processes take place under the influence of hemodynamic shear stress; therefore, we investigated how migrational speed and directionality are influenced by variations in shear stress. We examined human effector T lymphocytes on intercellular adhesion molecule 1 (ICAM-1)-coated surfaces under the influence of shear stresses from 2 to 60 dyn.cm(-2). T lymphocytes were shown to respond to shear stress application by a rapid (30 s) and fully reversible orientation of their migration against the fluid flow without a change in migration speed. Primary T lymphocytes migrating on ICAM-1 in the presence of uniformly applied SDF-1α were also found to migrate against the direction of shear flow. In sharp contrast, neutrophils migrating in the presence of uniformly applied fMLP and leukemic HSB2 T lymphocytes migrating on ICAM-1 alone oriented their migration downstream, with the direction of fluid flow. Our findings suggest that, in addition to biochemical cues, shear stress is a contributing factor to leukocyte migration directionality.
Cited literature [38 references]
https://www.hal.inserm.fr/inserm-00799746
Contributor : Marie-Pierre Valignat Connect in order to contact the contributor
Submitted on : Wednesday, January 22, 2014 - 7:00:12 AM
Last modification on : Sunday, November 28, 2021 - 4:38:02 PM
Long-term archiving on: : Sunday, April 2, 2017 - 11:40:18 AM
Contributor : Marie-Pierre Valignat Connect in order to contact the contributor
Submitted on : Wednesday, January 22, 2014 - 7:00:12 AM
Last modification on : Sunday, November 28, 2021 - 4:38:02 PM
Long-term archiving on: : Sunday, April 2, 2017 - 11:40:18 AM
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