Attachment of polysaccharides on the surface of nanoparticles offers possibility to modify physicochemical and biological properties of the core particles. Surface of lipid nanocapsules (LNC) was modified by post-insertion of amphiphilic lipochitosan (LC) or lipodextran (LD). Modelling of these LNC by drop tensiometer technique revealed that the positively charged LC made the LNC surface more rigid whereas the neutral, higher M(W) LD had no effect on the surface elasticity. Both LNC-LC and LNC-LD activated more the complement system than the blank LNC, thus proposing increased capture by the mononuclear phagocyte system (MPS). In vitro, the positively charged LNC-LC were more efficiently bound by the model HEK293(β(3)) cells compared to LNC and LNC-LD. Finally, it was observed that neither LC nor LD did change in vivo biodistribution properties of LNC in mice. These polysaccharide-coated LNC, especially LNC-LC, are promising templates for targeting ligands (e.g. peptides, proteins) or therapeutic molecules (e.g. siRNA).