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Journal articles
Repairing chronic myocardial infarction with autologous mesenchymal stem cells engineered tissue in rat promotes angiogenesis and limits ventricular remodeling.
Abstract : ABSTRACT: BACKGROUND: Tissue engineering scaffold constitutes a new strategy of myocardial repair. Here, we studied the contribution of a patch using autologous mesenchymal stem cells (MSCs) seeded on collagen-1 scaffold on the cardiac reconstruction in rat model of chronic myocardial infarction (MI). METHODS: Patches were cultured with controlled MSCs (growth, phenotype and potentiality). Twenty coronary ligated rats with tomoscingraphy (SPECT)-authenticated transmural chronic MI were referred into a control group (n = 10) and a treated group (n = 10) which beneficiated an epicardial MSC-patch engraftment. Contribution of MSC-patch was tested 1-mo after using non-invasive SPECT cardiac imaging, invasive hemodynamic assessment and immunohistochemistry. RESULTS: 3D-collagen environment affected the cell growth but not the cell phenotype and potentiality. MSC-patch integrates well the epicardial side of chronic MI scar. In treated rats, one-month SPECT data have documented an improvement of perfusion in MI segments compared to control (64 +/- 4% vs 49 +/- 3% p = 0.02) and a reduced infarction. Contractile parameter dp/dtmax and dp/dtmin were improved (p < 0.01). Histology showed an increase of ventricular wall thickness (1.75 +/- 0.24 vs 1.35 +/- 0.32 mm, p <0.05) and immunochemistry of the repaired tissue displayed enhanced angiogenesis and myofibroblast-like tissue. CONCLUSION: 3D-MSC-collagen epicardial patch engraftment contributes to reverse remodeling of chronic MI.
Cited literature [27 references]
https://www.hal.inserm.fr/inserm-00772079
Contributor : Ed. Bmc <>
Submitted on : Wednesday, January 9, 2013 - 9:08:31 PM
Last modification on : Friday, February 9, 2018 - 5:06:01 PM
Long-term archiving on: : Wednesday, April 10, 2013 - 3:56:44 AM
Contributor : Ed. Bmc <>
Submitted on : Wednesday, January 9, 2013 - 9:08:31 PM
Last modification on : Friday, February 9, 2018 - 5:06:01 PM
Long-term archiving on: : Wednesday, April 10, 2013 - 3:56:44 AM