Abstract : During the past two decades, two-photon microscopy has become a powerful tool in neuroscience. Unlike other imaging methods like MRI, its spatial resolution is micrometric and enables the observation of structures at the subcellular scale. In this chapter, the physical principles and the way to study phenomenon occurring in the living animal are summarized. Then, we describe the methods to observe the different components of the nervous system like neurons, glia and brain microvasculature. Finally, we discuss the advantages of two-photon microscopy in neuro-oncology and in the study of diseases.
http://www.hal.inserm.fr/inserm-00771945
Contributeur : Jean-Paul Issartel
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Soumis le : mercredi 9 janvier 2013 - 16:15:09
Dernière modification le : mardi 11 septembre 2018 - 11:00:02
Document(s) archivé(s) le : mercredi 10 avril 2013 - 03:54:53
Clément Ricard, Jonathan Coles, Raphaël Serduc, Boudewijn Van Der Sanden, Pascale Verant, et al.. Two-Photon Imaging. Larry R. Squire. Encyclopedia of Neuroscience, Academic press Elsevier, pp.1221-1229, 2009. 〈inserm-00771945〉
