On the use of Zn2+ to discriminate endonucleases activated during apoptosis. - Archive ouverte HAL Access content directly
Journal Articles Biochimie Year : 1997

On the use of Zn2+ to discriminate endonucleases activated during apoptosis.

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Abstract

One approach to discriminate among specific DNases in apoptosis is to use inhibitors specific for each endonuclease. Zn2+ is known to inhibit Ca(2+)- and Mg(2+)-dependent endonuclease enzymatic activities during apoptosis. Acidic DNases were thought to be insensitive to Zn2+. In this paper, we analyse the effects of Zn2+ on activity of DNase II, either purified or in nuclei from lens fiber cells. These cells follow a physiological nuclear degeneration with DNase II accumulation in their nuclei. We show that Zn2+ is able to inhibit also this acidic endonuclease at a concentration of 1-6 mM. At a higher concentration of Zn2+, DNA is extensively degraded during the assay, masking the inhibition of the enzyme. This DNA degradation in the presence of Zn2+ has led to an overestimation of the activity of DNase II in studies of apoptosis. Hence, Zn2+ cannot be used to specifically identify one endonuclease among the different DNases involved in nuclear degradation during programmed cell death.
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Dates and versions

inserm-00770541 , version 1 (07-01-2013)

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  • HAL Id : inserm-00770541 , version 1
  • PUBMED : 9352093

Cite

Alicia Torriglia, Elisabeth Chaudun, Yves Courtois, Marie-France Counis. On the use of Zn2+ to discriminate endonucleases activated during apoptosis.. Biochimie, 1997, 79 (7), pp.435-8. ⟨inserm-00770541⟩

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