Intramyocardial delivery of mesenchymal stem cell-seeded hydrogel preserves cardiac function and attenuates ventricular remodeling after myocardial infarction.

Eva Mathieu 1, 2 Guillaume Lamirault 1, 2, 3 Claire Toquet 4 Pierre Lhommet 1, 2 Emilie Rederstorff 2, 5 Sophie Sourice 2, 5 Kevin Biteau 2, 1 Philippe Hulin 2, 6 Virginie Forest 1, 2 Pierre Weiss 2, 3, 5 Jérôme Guicheux 2, 5 Patricia Lemarchand 1, 2, 3, *
* Auteur correspondant
1 ITX - unité de recherche de l'institut du thorax UMR1087 UMR6291
2 Structure Fédérative de Recherche Santé F. Bonamy
3 CHU Nantes - Centre hospitalier universitaire de Nantes
4 Service d'Anatomo-pathologie
5 LIOAD - Laboratoire d'ingénierie osteo-articulaire et dentaire
6 MicroPICell - Cellular and Tissular Imaging Core Facility of Nantes
Abstract : BACKGROUND: To improve the efficacy of bone marrow-derived mesenchymal stem cell (MSC) therapy targeted to infarcted myocardium, we investigated whether a self-setting silanized hydroxypropyl methylcellulose (Si-HPMC) hydrogel seeded with MSC (MSC+hydrogel) could preserve cardiac function and attenuate left ventricular (LV) remodeling during an 8-week follow-up study in a rat model of myocardial infarction (MI). METHODOLOGY/PRINCIPAL FINDING: Si-HPMC hydrogel alone, MSC alone or MSC+hydrogel were injected into the myocardium immediately after coronary artery ligation in female Lewis rats. Animals in the MSC+hydrogel group showed an increase in cardiac function up to 28 days after MI and a mid-term prevention of cardiac function alteration at day 56. Histological analyses indicated that the injection of MSC+hydrogel induced a decrease in MI size and an increase in scar thickness and ultimately limited the transmural extent of MI. These findings show that intramyocardial injection of MSC+hydrogel induced short-term recovery of ventricular function and mid-term attenuation of remodeling after MI. CONCLUSION/SIGNIFICANCE: These beneficial effects may be related to the specific scaffolding properties of the Si-HPMC hydrogel that may provide the ability to support MSC injection and engraftment within myocardium.
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PLoS ONE, Public Library of Science, 2012, 7 (12), pp.e51991. 〈10.1371/journal.pone.0051991〉
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Eva Mathieu, Guillaume Lamirault, Claire Toquet, Pierre Lhommet, Emilie Rederstorff, et al.. Intramyocardial delivery of mesenchymal stem cell-seeded hydrogel preserves cardiac function and attenuates ventricular remodeling after myocardial infarction.. PLoS ONE, Public Library of Science, 2012, 7 (12), pp.e51991. 〈10.1371/journal.pone.0051991〉. 〈inserm-00770250〉

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