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Cross sectional area (CSA) analysis of regenerated fibers in model of less severe muscle damage (10 -5 M CTX), showing increased myofiber size in Ad-sCripto treated mice compared to control. (D) Quantitative analysis of F4/80 positive cell area in mice treated with Ad-sCripto vs Ad-Control at day4 ,
9 ± 3% after Ad-Control at day 22; n=5 mice/group; P=NS; n=5 mice/group; P=NS) Values are mean ± SEM, 5 mice/time point. (E) PCR genotyping of TA muscle and bone marrow DNA from Tg:Pax7-CreERT2::Cripto loxP ,
GPI-anchored Cripto protein (mCripto) promotes myoblast proliferation and antagonizes Myostatin/GDF8 signaling pathway ,
Smad2 or -Cripto antibodies were used. (D) Expression of Cripto (E) Myostatin in TA muscles at day4, 8 and 22 after CTX injection, as shown by qRT-PCR. mRNA expression ,