Role of neurotrophins on dermal fibroblast survival and differentiation.

Abstract : Neurotrophins (NTs) belong to a family of growth factors that play a critical role in the control of skin homeostasis. NTs act through the low-affinity receptor p75NTR and the high-affinity receptors TrkA, TrkB, and TrkC. Here we show that dermal fibroblasts (DF) and myofibroblasts (DM) synthesize and secrete all NTs and express NT receptors. NTs induce differentiation of DF into DM, as shown by the expression of α-SMA protein. The Trk inhibitor K252a, TrkA/Fc, TrkB/Fc, or TrkC/Fc chimera prevents DF and DM proliferation. In addition, p75NTR siRNA inhibits DF proliferation, indicating that both NT receptors mediate DF proliferation induced by endogenous NTs. Autocrine NTs also induce DF migration through p75NTR and Trk, as either silencing of p75NTR or Trk/Fc chimeras prevent this effect, in absence of exogenous NTs. Finally, NGF or BDNF statistically increase the tensile strength in a dose dependent manner, as measured in a collagen gel through the GlaSbox device. Taken together, these results indicate that NTs exert a critical role on fibroblast and could be involved in tissue re-modeling and wound healing.
Type de document :
Article dans une revue
Journal of Cellular Physiology, Wiley, 2012, 227 (3), pp.1017-25. 〈10.1002/jcp.22811〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00769341
Contributeur : Philippe Saas <>
Soumis le : lundi 31 décembre 2012 - 13:18:58
Dernière modification le : lundi 9 juillet 2018 - 13:12:02

Lien texte intégral

Identifiants

Collections

Citation

Elisabetta Palazzo, Alessandra Marconi, Francesca Truzzi, Katiuscia Dallaglio, Tiziana Petrachi, et al.. Role of neurotrophins on dermal fibroblast survival and differentiation.. Journal of Cellular Physiology, Wiley, 2012, 227 (3), pp.1017-25. 〈10.1002/jcp.22811〉. 〈inserm-00769341〉

Partager

Métriques

Consultations de la notice

134