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Use of heparin matrix for detecting heparin-binding growth factor receptors in detergent soluble fractions: its application to identification of a fibroblast growth factor receptor in retina.

Abstract : Classical cross-linking technology has been found to be of very limited use in identifying solubilized receptors. A new assay based on the use of heparin-Sepharose was developed here for detecting receptors of fibroblast growth factors (FGFs) in detergent-soluble fractions. This new cross-linking assay is based on the heparin-binding properties of FGF. To this end, solubilized FGF receptors were cross-linked to radiolabelled acidic FGF on heparin-Sepharose. Standardization of the method was carried out by using: 1) crude CHAPS-soluble extracts from chinese hamster ovary cells which express human FGF receptor cDNA (FGFR-2); 2) CHAPS-soluble fractions purified by affinity chromatography from bovine retina. This approach might be a valuable tool for detecting receptors of all heparin-binding growth factors.
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https://www.hal.inserm.fr/inserm-00767958
Contributor : Alicia Torriglia Connect in order to contact the contributor
Submitted on : Thursday, December 20, 2012 - 4:15:36 PM
Last modification on : Friday, December 21, 2012 - 5:08:35 PM

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Alicia Torriglia, Pierre R. Blanquet. Use of heparin matrix for detecting heparin-binding growth factor receptors in detergent soluble fractions: its application to identification of a fibroblast growth factor receptor in retina.. Analytical Letters, Taylor & Francis, 1993, 26 (1), pp.33-47. ⟨10.1080/00032719308016793⟩. ⟨inserm-00767958⟩

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