Use of heparin matrix for detecting heparin-binding growth factor receptors in detergent soluble fractions: its application to identification of a fibroblast growth factor receptor in retina. - Archive ouverte HAL Access content directly
Journal Articles Analytical Letters Year : 1993

Use of heparin matrix for detecting heparin-binding growth factor receptors in detergent soluble fractions: its application to identification of a fibroblast growth factor receptor in retina.

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Alicia Torriglia
  • Function : Author
  • PersonId : 913045
Pierre R. Blanquet
  • Function : Author

Abstract

Classical cross-linking technology has been found to be of very limited use in identifying solubilized receptors. A new assay based on the use of heparin-Sepharose was developed here for detecting receptors of fibroblast growth factors (FGFs) in detergent-soluble fractions. This new cross-linking assay is based on the heparin-binding properties of FGF. To this end, solubilized FGF receptors were cross-linked to radiolabelled acidic FGF on heparin-Sepharose. Standardization of the method was carried out by using: 1) crude CHAPS-soluble extracts from chinese hamster ovary cells which express human FGF receptor cDNA (FGFR-2); 2) CHAPS-soluble fractions purified by affinity chromatography from bovine retina. This approach might be a valuable tool for detecting receptors of all heparin-binding growth factors.
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inserm-00767958 , version 1 (20-12-2012)

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Alicia Torriglia, Pierre R. Blanquet. Use of heparin matrix for detecting heparin-binding growth factor receptors in detergent soluble fractions: its application to identification of a fibroblast growth factor receptor in retina.. Analytical Letters, 1993, 26 (1), pp.33-47. ⟨10.1080/00032719308016793⟩. ⟨inserm-00767958⟩

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