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Magnetic resonance imaging and fluorescence labeling of clinical-grade mesenchymal stem cells without impacting their phenotype: study in a rat model of stroke.

Abstract : Human mesenchymal stem cells (hMSCs) have strong potential for cell therapy after stroke. Tracking stem cells in vivo following a graft can provide insight into many issues regarding optimal route and/or dosing. hMSCs were labeled for magnetic resonance imaging (MRI) and histology with micrometer-sized superparamagnetic iron oxides (M-SPIOs) that contained a fluorophore. We assessed whether M-SPIO labeling obtained without the use of a transfection agent induced any cell damage in clinical-grade hMSCs and whether it may be useful for in vivo MRI studies after stroke. M-SPIOs provided efficient intracellular hMSC labeling and did not modify cell viability, phenotype, or in vitro differentiation capacity. Following grafting in a rat model of stroke, labeled hMSCs could be detected using both in vivo MRI and fluorescent microscopy until 4 weeks following transplantation. However, whereas good label stability and unaffected hMSC viability were observed in vitro, grafted hMSCs may die and release iron particles in vivo.
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https://www.hal.inserm.fr/inserm-00760939
Contributor : Michel Dojat <>
Submitted on : Tuesday, December 4, 2012 - 3:53:10 PM
Last modification on : Friday, November 6, 2020 - 4:07:05 AM
Long-term archiving on: : Saturday, December 17, 2016 - 7:54:12 PM

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Olivier Detante, Samuel Valable, Florence de Fraipont, Emmanuelle Grillon, Emmanuel Luc Barbier, et al.. Magnetic resonance imaging and fluorescence labeling of clinical-grade mesenchymal stem cells without impacting their phenotype: study in a rat model of stroke.. Stem Cells Transl Med, 2012, 1 (4), pp.333-41. ⟨10.5966/sctm.2011-0043⟩. ⟨inserm-00760939⟩

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