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Journal articles
Magnetic resonance imaging and fluorescence labeling of clinical-grade mesenchymal stem cells without impacting their phenotype: study in a rat model of stroke.
Olivier Detante
1, *
Samuel Valable
2
Florence de Fraipont
3, 4
Emmanuelle Grillon
5
Emmanuel Luc Barbier
5
Anaïck Moisan
5
Josiane Arnaud
6
Christine Moriscot
4, 7
Christoph Segebarth
5
Marc Hommel
8
Chantal Rémy
5
Marie-Jeanne Richard
3, 4
*
Corresponding author
1
INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale
GIN - Grenoble Institut des Neurosciences, Unité neuro-vascularie, neurologie
5
ANTE-INSERM U836, équipe 5 - Neuro-imagerie fonctionnelle et métabolique
GIN - Grenoble Institut des Neurosciences
Olivier Detante 1
Correspondent author
Samuel Valable 2
Author IdHAL : samuel-valable ResearcherId : http://www.researcherid.com/rid/B-7578-2008 ORCID : https://orcid.org/0000-0003-0355-0270
Florence de Fraipont 3, 4
Author
Emmanuelle Grillon 5
Author
Emmanuel Luc Barbier 5
Author IdHAL : emmanuel-barbier ResearcherId : http://www.researcherid.com/rid/E-6302-2011 ORCID : https://orcid.org/0000-0002-4952-1240
Anaïck Moisan 5
Author
Josiane Arnaud 6
Author
Christine Moriscot 4, 7
Author
Christoph Segebarth 5
Author
Marc Hommel 8
Author
Chantal Rémy 5
Author
Marie-Jeanne Richard 3, 4
Author
1
INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale (France)
- GIN - Grenoble Institut des Neurosciences (UJF - Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9 - France)
- UJF - Université Joseph Fourier - Grenoble 1 (BP 53 - 38041 Grenoble Cedex 9 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U836 (101, rue de Tolbiac, 75013 Paris - France)
- Unité neuro-vascularie, neurologie (Grenoble - France)
- CHU Grenoble (France)
2
GIN - Grenoble Institut des Neurosciences (UJF - Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9 - France)
- UJF - Université Joseph Fourier - Grenoble 1 (BP 53 - 38041 Grenoble Cedex 9 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U836 (101, rue de Tolbiac, 75013 Paris - France)
3
INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble (Institut Albert Bonniot, BP170, 38042 Grenoble Cedex 9 - France)
- UJF - Université Joseph Fourier - Grenoble 1 (BP 53 - 38041 Grenoble Cedex 9 - France)
- CHU Grenoble (France)
- EFS (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U823 (101, rue de Tolbiac, 75013 Paris - France)
5
ANTE-INSERM U836, équipe 5 - Neuro-imagerie fonctionnelle et métabolique (Chemin Fortuné Ferrini
38700 La Tronche - France)
- GIN - Grenoble Institut des Neurosciences (UJF - Site Santé La Tronche - BP 170 - 38042 Grenoble Cedex 9 - France)
- UJF - Université Joseph Fourier - Grenoble 1 (BP 53 - 38041 Grenoble Cedex 9 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U836 (101, rue de Tolbiac, 75013 Paris - France)
6
Biochimie Hormonale et Nutritionnelle (France)
- UJF - Université Joseph Fourier - Grenoble 1 (BP 53 - 38041 Grenoble Cedex 9 - France)
- CHU Grenoble (France)
8
CIC - Grenoble (Centre Hospitalier Universitaire LYON 38043 GRENOBLE CEDEX 9 - France)
- UJF - Université Joseph Fourier - Grenoble 1 (BP 53 - 38041 Grenoble Cedex 9 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : CIC3 (101, rue de Tolbiac, 75013 Paris - France)
Abstract : Human mesenchymal stem cells (hMSCs) have strong potential for cell therapy after stroke. Tracking stem cells in vivo following a graft can provide insight into many issues regarding optimal route and/or dosing. hMSCs were labeled for magnetic resonance imaging (MRI) and histology with micrometer-sized superparamagnetic iron oxides (M-SPIOs) that contained a fluorophore. We assessed whether M-SPIO labeling obtained without the use of a transfection agent induced any cell damage in clinical-grade hMSCs and whether it may be useful for in vivo MRI studies after stroke. M-SPIOs provided efficient intracellular hMSC labeling and did not modify cell viability, phenotype, or in vitro differentiation capacity. Following grafting in a rat model of stroke, labeled hMSCs could be detected using both in vivo MRI and fluorescent microscopy until 4 weeks following transplantation. However, whereas good label stability and unaffected hMSC viability were observed in vitro, grafted hMSCs may die and release iron particles in vivo.
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Journal articles
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https://www.hal.inserm.fr/inserm-00760939
Contributor : Michel Dojat Connect in order to contact the contributor
Submitted on : Tuesday, December 4, 2012 - 3:53:10 PM
Last modification on : Thursday, May 20, 2021 - 4:50:02 PM
Long-term archiving on: : Saturday, December 17, 2016 - 7:54:12 PM
Contributor : Michel Dojat Connect in order to contact the contributor
Submitted on : Tuesday, December 4, 2012 - 3:53:10 PM
Last modification on : Thursday, May 20, 2021 - 4:50:02 PM
Long-term archiving on: : Saturday, December 17, 2016 - 7:54:12 PM
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Identifiers
- HAL Id : inserm-00760939, version 1
- DOI : 10.5966/sctm.2011-0043
- PUBMED : 23197812
Citation
Olivier Detante, Samuel Valable, Florence de Fraipont, Emmanuelle Grillon, Emmanuel Luc Barbier, et al.. Magnetic resonance imaging and fluorescence labeling of clinical-grade mesenchymal stem cells without impacting their phenotype: study in a rat model of stroke.. Stem Cells Transl Med, 2012, 1 (4), pp.333-41. ⟨10.5966/sctm.2011-0043⟩. ⟨inserm-00760939⟩
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