Imaging submillisecond membrane potential changes from individual regions of single axons, dendrites and spines - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Book Sections Year : 2011

Imaging submillisecond membrane potential changes from individual regions of single axons, dendrites and spines

Abstract

A central question in neuronal network analysis is how the interaction between individual neurons produces behavior and behavioral modifications. This task depends critically on how exactly are signals integrated by individual nerve cells functioning as complex operational units. Regional electrical properties of branching neuronal processes which determine the input-output function of any neuron are extraordinarily complex, dynamic, and, in the general case, impossible to predict in the absence of detailed measurements. To obtain such a measurement one would, ideally, like to be able to monitor, at multiple sites, subthreshold events as they travel from the sites of origin (synaptic contacts on distal dendrites) and summate at particular locations to influence action potential initiation. It became possible recently to carry out this type of measurement using high-resolution multisite recording of membrane potential changes with intracellular voltage-sensitive dyes. This chapter reviews the development and foundation of the method of voltage-sensitive dye recording from individual neurons. Presently, this approach allows monitoring membrane potential transients from all parts of the dendritic tree as well as from axon collaterals and individual dendritic spines.
Embargoed file
Embargoed file
Ne sera jamais visible
Loading...

Dates and versions

inserm-00759036 , version 1 (29-11-2012)

Identifiers

  • HAL Id : inserm-00759036 , version 1

Cite

Marco Canepari, Marko Popović, Kaspar E. Vogt, Knut Holthoff, Arthur Konnerth, et al.. Imaging submillisecond membrane potential changes from individual regions of single axons, dendrites and spines. Canepari, M; Zecevic, D. Menbrane potential imaging in the nervous system: methods and applications, Springer, pp.25-41, 2011. ⟨inserm-00759036⟩

Collections

INSERM U836
88 View
1 Download

Share

Gmail Facebook Twitter LinkedIn More