1479-5876-10-S3-O41479-5876 Oral presentation <p>No lack of regulatory B cells in patients with Multiple Sclerosis</p> MichelLaure ChesneauMélanie ManceauPhilippe GarciaAlexandra SalouMarion NgonoElongAnnie PallierAnnaïck Jacq-FoucherMarylène LefrèreFabienne WiertlewskiSandrine SoulillouJean-Paul DegauqueNicolas LaplaudDavid-Axel BrouardSophie

INSERM, UMR 1064, Nantes, France

CHU Nord Laennec, Service de Neurologie, Nantes, France

CHU de Nantes, ITUN, Nantes, France

 Journal of Translational Medicine <p>7th European Workshop on Immune-Mediated Inflammatory Diseases</p>Timothy Radstake, Leonie Taams and Ola WinqvistMeeting abstracts<p>7th European Workshop on Immune-Mediated Inflammatory Diseases</p>Noordwijk aan Zee, the Netherlands28-30 November 2012http://www.ewimid.com/1479-5876 2012 10 Suppl 3 O4 http://www.translational-medicine.com/content/10/S3/O4 10.1186/1479-5876-10-S3-O4 28112012 2012Michel et al; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

Recent data support a prominent role for B cells in MS physiopathology. Recently it has emerged that subsets of B cells secreting IL-10 negatively regulate disease symptoms in Experimental Autoimmune Encephalomyelitis (EAE). However, the involvement of such regulatory B cells in MS remains unclear.

Aim

We aimed to study the frequency, phenotype and function of regulatory B cells in MS patients as compared to Healthy Volunteers (HV).

Methods

Sixty-three untreated MS patients and 58 HV were included in this study. IL-10 secretion by B cells and phenotype of IL-10+ B cells were studied after 5h (B10 cells) and 48h of stimulation (B10pro cells) by CD40L and ODN. Coculture assays with prestimulated B cells and responding CD4+CD25- T cells were performed for 3 days.

Results

No significant difference was found either for IL-10 secretion ability of B cells after 5h or 48h of stimulation. The analysis of B10pro cells phenotype revealed mainly a memory phenotype in MS and HV, even if both naïve and immature subsets were also able to secrete IL-10. Prestimulated B cells from MS inhibited CD4+CD25- T cell proliferation in the same manner than HV by a contact dependent mechanism, independently of IL-10 and TGF-β secretion.

Conclusion

Altogether, our data show that regulatory B cells have a conserved frequency, phenotype and function in the blood of patients with MS suggesting that B cells do not contribute to the physiopathology of the disease.

Acknowledgements

This work was funded by ARSEP.