Chronic ethanol exposure increases gene transcription of subunits of an N-methyl-D-aspartate receptor-like complex in cortical neurons in culture.

Xiaodong Bao 1, 2 Dongwei Hui 1, 2 Mickaël Naassila 1, 2 Elias Michaelis 1, 2
1 Department of Pharmacology and Toxicology
2 The Center for Neurobiology and Immunology Research
Abstract : Chronic exposure to ethanol leads to adaptive responses in neurons, including enhanced activity of N-methyl-D-aspartate (NMDA) receptors. Chronic treatment of neurons with ethanol also leads to increases in the protein levels of three subunits of an NMDA receptor-like complex. In the present study, we explored whether the increases in subunit protein levels are the result of either increased transcription or diminished protein turnover. We found that a 72-h exposure of cortical neurons in culture to 100 mM ethanol caused enhanced transcription of the genes for the glutamate-binding (GBP) and glycine-binding protein subunits of the receptor-like complex. This treatment had no effect on protein turnover of either GBP or of the NMDA receptor subunit NR1, suggesting that the increases in protein expression are the result of increased DNA transcription.
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Neuroscience Letters, Elsevier, 2001, 315 (1-2), pp.5-8
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http://www.hal.inserm.fr/inserm-00746182
Contributeur : Mickael Naassila <>
Soumis le : samedi 27 octobre 2012 - 19:48:46
Dernière modification le : vendredi 9 mars 2018 - 13:54:02

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  • HAL Id : inserm-00746182, version 1
  • PUBMED : 11711201

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Xiaodong Bao, Dongwei Hui, Mickaël Naassila, Elias Michaelis. Chronic ethanol exposure increases gene transcription of subunits of an N-methyl-D-aspartate receptor-like complex in cortical neurons in culture.. Neuroscience Letters, Elsevier, 2001, 315 (1-2), pp.5-8. 〈inserm-00746182〉

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