Manganese enhanced MRI in rat hippocampus: A correlative study with synchrotron X-ray microprobe. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue NeuroImage Année : 2012

Manganese enhanced MRI in rat hippocampus: A correlative study with synchrotron X-ray microprobe.

Résumé

Manganese enhanced MRI (MEMRI) offers many possibilities such as tract tracing and functional imaging in vivo. Mn is however neurotoxic and may induce symptoms similar to those associated with Parkinson's disease (manganism). The mechanisms of Mn-induced neurotoxicity are not clear. In this study, we combine synchrotron X-ray fluorescence microprobe (SR-XRF) and MEMRI techniques to investigate spatial distribution of Mn within the rat hippocampus and how Mn interacts with Ca, Fe and Zn at a cellular level. Images were acquired in the rat hippocampus (n=23) and using two injection routes: intra-cerebral (MnCl(2): 50mM, 10μL) and intra-peritoneal (MnCl(2): 100mM, 30mg/kg). For both injection routes, Mn is found in dentate gyrus and in CA3: control: 2.5±1.6, intra-peritoneal: 5.0±2.4, and intra-cerebral: 25.1±9.2μg/g. Mn follows Zn distribution and has a negative impact on the total amount of Zn and Fe. The Mn-enhanced MRI contrast is well correlated with the total Mn amount measured with SR-XRF (R(2)=0.93; p<0.002). After intra-cerebral injection, the hippocampal fissure is found to accumulate a large amount of Mn and yields a hypointense MRI signal, which may be ascribed to a reduction in T2. This study shows that SR-XRF is well suited to investigate Mn distribution at a mesoscale and that MRI is sensitive to low Mn concentrations. As perturbations in metal homeostasis may alter brain function, the injected dose of Mn in MEMRI studies needs to be carefully adjusted to obtain reliable functional information.
Fichier principal
Vignette du fichier
Daoust_2012_ManganA_se_MA.PDF (1022.17 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

inserm-00744958 , version 1 (24-10-2012)

Identifiants

Citer

Alexia Daoust, Emmanuel L. Barbier, Sylvain Bohic. Manganese enhanced MRI in rat hippocampus: A correlative study with synchrotron X-ray microprobe.. NeuroImage, 2012, 64C, pp.10-18. ⟨10.1016/j.neuroimage.2012.09.025⟩. ⟨inserm-00744958⟩

Collections

INSERM UGA U836
271 Consultations
772 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More