Specific in vivo staining of astrocytes in the whole brain after intravenous injection of sulforhodamine dyes.

Florence Appaix 1, * Sabine Girod 2 Sylvie Boisseau 3 Johannes Römer 1 Jean-Claude Vial 4 Mireille Albrieux 3 Mathieu Maurin 1 Antoine Depaulis 2 Isabelle Guillemain 2 Boudewijn Van Der Sanden 5
* Auteur correspondant
1 GIN - Grenoble Institut des Neurosciences
2 INSERM U836, équipe 9, Dynamique des réseaux synchrones épileptiques
GIN - Grenoble Institut des Neurosciences
3 INSERM U836, équipe 10, Dynamique des réseaux neuronaux du mouvement
GIN - Grenoble Institut des Neurosciences
4 LSP - Laboratoire de Spectrométrie Physique
5 Inserm U836, équipe 7, Nanomédecine et cerveau
GIN - Grenoble Institut des Neurosciences
Abstract : Fluorescent staining of astrocytes without damaging or interfering with normal brain functions is essential for intravital microscopy studies. Current methods involved either transgenic mice or local intracerebral injection of sulforhodamine 101. Transgenic rat models rarely exist, and in mice, a backcross with GFAP transgenic mice may be difficult. Local injections of fluorescent dyes are invasive. Here, we propose a non-invasive, specific and ubiquitous method to stain astrocytes in vivo. This method is based on iv injection of sulforhodamine dyes and is applicable on rats and mice from postnatal age to adulthood. The astrocytes staining obtained after iv injection was maintained for nearly half a day and showed no adverse reaction on astrocytic calcium signals or electroencephalographic recordings in vivo. The high contrast of the staining facilitates the image processing and allows to quantify 3D morphological parameters of the astrocytes and to characterize their network. Our method may become a reference for in vivo staining of the whole astrocytes population in animal models of neurological disorders.
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Article dans une revue
PLoS ONE, Public Library of Science, 2012, 7 (4), pp.e35169. 〈10.1371/journal.pone.0035169〉
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Florence Appaix, Sabine Girod, Sylvie Boisseau, Johannes Römer, Jean-Claude Vial, et al.. Specific in vivo staining of astrocytes in the whole brain after intravenous injection of sulforhodamine dyes.. PLoS ONE, Public Library of Science, 2012, 7 (4), pp.e35169. 〈10.1371/journal.pone.0035169〉. 〈inserm-00738160〉

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