Bmcc1s, a novel brain-isoform of Bmcc1, affects cell morphology by regulating MAP6/STOP functions.

Jessica Arama; Anne-Cécile Boulay; Christophe Bosc; Christian Delphin; Damarys Loew; Phillipe Rostaing; Edwige Amigou; Pascal Ezan; Laure Wingertsmann; Laurent Guillaud; Annie Andrieux; Christian Giaume; Martine Cohen-Salmon

doi:10.1371/journal.pone.0035488

Bmcc1s, a novel brain-isoform of Bmcc1, affects cell morphology by regulating MAP6/STOP functions.

Jessica Arama ^{1, 2, 3} Anne-Cécile Boulay ^{1, 2, 3} Christophe Bosc ⁴ Christian Delphin ⁴ Damarys Loew ⁵ Phillipe Rostaing ⁶ Edwige Amigou ^{1, 2, 3} Pascal Ezan ^{1, 2, 3} Laure Wingertsmann ^{6, 7} Laurent Guillaud ⁸ Annie Andrieux ⁹ Christian Giaume ^{1, 2, 3} Martine Cohen-Salmon ^{1, 2, 3, *}

* Auteur correspondant

1 CIRB - Centre interdisciplinaire de recherche en biologie

2 UPMC - Université Pierre et Marie Curie - Paris 6

3 MEMOLIFE Laboratoire d'excellence de Paris Sciences et Lettres

4 INSERM U836, équipe 1, Physiopathologie du cytosquelette

GIN - Grenoble Institut des Neurosciences

5 Laboratoire de Spectrométrie de Masse Protéomique

6 IBENS - Institut de biologie de l'Ecole Normale Supérieure

7 IBENS - Institut de biologie de l'ENS Paris (UMR 8197/1024)

8 Cell and Molecular Synaptic Function Unit

9 INSERM U836, équipe 1, Physiopathologie du cytosquelette

GPC-GIN - Groupe Physiopathologie du Cytosquelette, GIN - Grenoble Institut des Neurosciences

Abstract : The BCH (BNIP2 and Cdc42GAP Homology) domain-containing protein Bmcc1/Prune2 is highly enriched in the brain and is involved in the regulation of cytoskeleton dynamics and cell survival. However, the molecular mechanisms accounting for these functions are poorly defined. Here, we have identified Bmcc1s, a novel isoform of Bmcc1 predominantly expressed in the mouse brain. In primary cultures of astrocytes and neurons, Bmcc1s localized on intermediate filaments and microtubules and interacted directly with MAP6/STOP, a microtubule-binding protein responsible for microtubule cold stability. Bmcc1s overexpression inhibited MAP6-induced microtubule cold stability by displacing MAP6 away from microtubules. It also resulted in the formation of membrane protrusions for which MAP6 was a necessary cofactor of Bmcc1s. This study identifies Bmcc1s as a new MAP6 interacting protein able to modulate MAP6-induced microtubule cold stability. Moreover, it illustrates a novel mechanism by which Bmcc1 regulates cell morphology.

Type de document :

Article dans une revue

PLoS ONE, Public Library of Science, 2012, 7 (4), pp.e35488. 〈10.1371/journal.pone.0035488〉

Domaine :

Sciences du Vivant [q-bio] / Neurosciences [q-bio.NC]

Sciences du Vivant [q-bio] / Biologie cellulaire

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http://www.hal.inserm.fr/inserm-00734621
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Bmcc1s, a novel brain-isoform of Bmcc1, affects cell morphology by regulating MAP6/STOP functions.

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