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Influence of sildenafil on micturition and urethral tone in ovariectomized and non-ovariectomized mice.

Abstract : INTRODUCTION: Nitric oxide synthases (NOSs) and estrogen receptors are expressed in the female urethra. AIM: We aimed to assess the impact of sildenafil on micturition behavior, urethral tone according to the hormonal status and to determine the implications of the neuronal isoform of NOS (nNOS). METHODS: Four-week-old C57/BL6 female mice were sham-operated or ovariectomized. Six weeks later, they were injected intraperitoneally by any combination of sildenafil, 7-nitroindazole (7-NI)-a potent selective nNOS inhibitor-or the corresponding vehicles. The mice were then subjected to micturition behavior and leak point pressure studies. Urethral histomorphometry was performed. MAIN OUTCOME MEASURES: The main outcome measures were micturition behavior, leak point pressure, and histomorphometry. RESULTS: In sham-operated and ovariectomized animals, sildenafil did not impact micturition, although it decreased urethral resistance 10-fold. nNOS inhibition by 7-NI reduced the number of micturitions and increased residual volume and leak point pressure. It abrogated sildenafil-induced drop in urethral resistances. Hormonal status did not influence the structure of the urethral layers. CONCLUSIONS: Irrespective of the hormonal status, sildenafil decreased leak point pressure by a nNOS-mediated mechanism.
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Contributor : Marie Francoise Simon Connect in order to contact the contributor
Submitted on : Friday, September 14, 2012 - 2:04:02 PM
Last modification on : Wednesday, June 1, 2022 - 4:05:30 AM

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Xavier Gamé, Ourdia Bouali, Julien Allard, Pierre Gourdy, Ghislaine Escourrou, et al.. Influence of sildenafil on micturition and urethral tone in ovariectomized and non-ovariectomized mice.. Journal of Sexual Medicine, Wiley, 2012, 9 (2), pp.466-71. ⟨10.1111/j.1743-6109.2011.02559.x⟩. ⟨inserm-00732310⟩



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