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C. Cells, C. +. Cd25, and T. Eff, were cultured for 5 days in wells coated with anti-CD3 (1µg/ml) in the presence of Notch ligands (5 µg/ml) or IgG control at different ratios. Percentages of inhibition were calculated from the raw data using the equations: 1-(mean cpm of co-culture wells divided by mean cpm of CD4 + CD25 -cells cultured alone) x 100