Skip to Main content Skip to Navigation
Journal articles

The NF-κB member p65 controls glutamine metabolism through miR-23a.

Abstract : Cancer cells have elevated aerobic glycolysis that is termed the Warburg effect. But several tumor cells, including leukemic cells, also increase glutamine metabolism, which is initiated by glutaminase (GLS). The microRNA (miRNA) miR-23 targets GLS mRNA and inhibits expression of GLS protein. Here we show that in human leukemic Jurkat cells the NF-κB p65 subunit binds to miR-23a promoter and inhibits miR-23a expression. Histone deacetylase (HDAC) inhibitors release p65-induced inhibition. Jurkat cells growing in glutamine decrease proliferation due to cell accumulation in G0/G1 phase. Nevertheless, cells get used to this new source of energy by increasing GLS expression, which correlates with an increase in p65 expression and its translocation to the nucleus, leading to a higher basal NF-κB activity. Jurkat cells overexpressing p65 show increase basal GLS expression and proliferate faster than control cells in glutamine medium. Overexpressing miR-23a in leukemic cells impaired glutamine use and induces mitochondrial dysfunction leading to cell death. Therefore, p65 activation decreases miR-23a expression, which facilitates glutamine consumption allowing leukemic cells to use this alternative source of carbon and favoring their adaptation to the metabolic environment.
Document type :
Journal articles
Complete list of metadata

Cited literature [43 references]  Display  Hide  Download
Contributor : Monique Frei Connect in order to contact the contributor
Submitted on : Friday, August 31, 2012 - 9:34:55 AM
Last modification on : Tuesday, May 17, 2022 - 2:20:01 PM
Long-term archiving on: : Friday, March 31, 2017 - 12:10:43 PM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document



Moeez G. Rathore, Anne Saumet, Jean-François Rossi, Carine de Bettignies, Denis Tempé, et al.. The NF-κB member p65 controls glutamine metabolism through miR-23a.. International Journal of Biochemistry and Cell Biology, Elsevier, 2012, 44 (9), pp.1448-56. ⟨10.1016/j.biocel.2012.05.011⟩. ⟨inserm-00726691⟩



Record views