Identification of adipose tissue dendritic cells correlated with obesity-associated insulin-resistance and inducing th17 responses in mice and patients.

Adeline Bertola 1 Thomas Ciucci 2 Déborah Rousseau 1 Virginie Bourlier 3 Carine Duffaut 3 Stéphanie Bonnafous 1, 4 Claudine Blin-Wakkach 2 Rodolphe Anty 1, 4 Antonio Iannelli 1, 4 Jean Gugenheim 1, 4 Albert Tran 1, 4 Anne Bouloumié 3 Philippe Gual 4, 1, * Abdelilah Wakkach 2
* Auteur correspondant
1 C3M - Centre méditérannéen de médecine moléculaire
2 LP2M - Laboratoire de PhysioMédecine Moléculaire
3 I2MC - Institut des Maladies Métaboliques et Cardiovasculaires
4 Département Digestif
Abstract : T-cell regulation in adipose tissue provides a link between inflammation and insulin resistance. Because of alterations in adipose tissue T-cell composition in obesity, we aimed to identify the antigen-presenting cells in adipose tissue of obese mice and patients with insulin resistance. Dendritic cells (DCs) and T cells were studied in mice and in two cohorts of obese patients. In lean mice, only CD11c(+) DCs were detected in adipose tissue. Adoptive transfer of naive CD4(+) T cells in Rag1(-/-) mice led to a predominant Th1 response in adipose tissue. In contrast, during obesity DCs (human CD11c(+)CD1c(+) and mouse CD11c(high)F4/80(low)) accumulated in adipose tissue. CD11c(high)F4/80(low) DCs from obese mice induced Th17 differentiation. In patients, the presence of CD11c(+)CD1c(+) DCs correlated with the BMI and with an elevation in Th17 cells. In addition, these DCs led to ex vivo Th17 differentiation. CD1c gene expression further correlated with homeostatic model assessment-insulin resistance in the subcutaneous adipose tissue of obese patients. We show for the first time the presence and accumulation of specific DCs in adipose tissue in mouse and human obesity. These DCs were functional and could be important regulators of adipose tissue inflammation by regulating the switch toward Th17 cell responses in obesity-associated insulin resistance.
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Article dans une revue
Diabetes, American Diabetes Association, 2012, 61 (9), pp.2238-47. 〈10.2337/db11-1274〉
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http://www.hal.inserm.fr/inserm-00726176
Contributeur : Marie Francoise Simon <>
Soumis le : mercredi 29 août 2012 - 11:03:23
Dernière modification le : jeudi 4 octobre 2018 - 09:50:09

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UNICE | UNIV-TLSE3 | UNIV-COTEDAZUR

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Adeline Bertola, Thomas Ciucci, Déborah Rousseau, Virginie Bourlier, Carine Duffaut, et al.. Identification of adipose tissue dendritic cells correlated with obesity-associated insulin-resistance and inducing th17 responses in mice and patients.. Diabetes, American Diabetes Association, 2012, 61 (9), pp.2238-47. 〈10.2337/db11-1274〉. 〈inserm-00726176〉

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