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The gut microbiota profile is associated with insulin action in humans.

Abstract : The role of the gut microbiota in the induction of metabolic diseases has now been increasingly recognized worldwide. Indeed, a specific gut microbiota has been shown to characterize lean versus obese phenotypes both in humans and mice. We have also recently demonstrated that a precise gut microbiota is associated with the host's responsiveness to a high-fat diet. Therefore, we hypothesized that insulin resistance in humans could also be linked to a specific gut microbiota. To this aim, microbial DNA and RNA were extracted from the appendix contents of insulin-resistant versus insulin-sensitive obese subjects, matched for body mass index and age, and analyzed by DNA- and RNA-DGGE. Microbial DNA analysis showed that the patients fully segregated according to their degree of insulin action. Conversely, microbial RNA investigation showed that some degree of homology still existed between insulin-sensitive and insulin-resistant patients. Quantitative trait analysis, ordinary least squares regression, principal components regression, partial least squares, canonical correlation analysis, and canonical correspondence analysis also showed a net separation of the two phenotypes analyzed. We conclude that a specific gut microbial profile is associated with insulin action in humans.
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Submitted on : Wednesday, August 29, 2012 - 1:42:36 PM
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Matteo Serino, José Manuel Fernández-Real, Eduardo García Fuentes, Maribel Queipo-Ortuño, José María Moreno-Navarrete, et al.. The gut microbiota profile is associated with insulin action in humans.. Acta Diabetologica, Springer Verlag, 2013, 50 (5), pp.753-61. ⟨10.1007/s00592-012-0410-5⟩. ⟨inserm-00726167⟩



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