Skip to Main content Skip to Navigation
Journal articles

Resected RNA pseudoknots and their recognition by histidyl-tRNA synthetase.

Abstract : Duplexes constituted by closed or open RNA circles paired to single-stranded oligonucleotides terminating with 3'-CCAOH form resected pseudoknots that are substrates of yeast histidyl-tRNA synthetase. Design of this RNA fold is linked to the mimicry of the pseudoknotted amino acid accepting branch of the tRNA-like domain from brome mosaic virus, known to be charged by tyrosyl-tRNA synthetases, with RNA minihelices recapitulating accepting branches of canonical tRNAs. Prediction of the histidylation function of the new family of minimalist tRNA-like structures relates to the geometry of resected pseudoknots that allows proper presentation to histidyl-tRNA synthetase of analogues of the histidine identity determinants N-1 and N73 present in tRNAs. This geometry is such that the analogue of the major N-1 histidine determinant in the RNA circles faces the analogue of the discriminator N73 nucleotide in the accepting oligonucleotides. The combination of identity elements found in tRNAHis species from archaea, eubacteria, and organelles (G-1/C73) is the most efficient for determining histidylation of the duplexes. The inverse combination (C-1/G73) leads to the worst histidine acceptors with charging efficiencies reduced by 2-3 orders of magnitude. Altogether, these findings open new perspectives for understanding evolution of tRNA identity and serendipitous RNA functions.
Document type :
Journal articles
Complete list of metadatas

Cited literature [25 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00719607
Contributor : Annick Lefevre <>
Submitted on : Friday, July 20, 2012 - 11:50:56 AM
Last modification on : Tuesday, May 28, 2019 - 5:08:06 PM
Long-term archiving on: : Friday, December 16, 2016 - 1:31:12 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

  • HAL Id : inserm-00719607, version 1
  • PUBMED : 9724720

Collections

Citation

Brice Felden, Richard Giegé. Resected RNA pseudoknots and their recognition by histidyl-tRNA synthetase.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 1998, 95 (18), pp.10431-6. ⟨inserm-00719607⟩

Share

Metrics

Record views

134